Scheek R M, Boelens R, Russo N, van Boom J H, Kaptein R
Biochemistry. 1984 Mar 27;23(7):1371-6. doi: 10.1021/bi00302a006.
A sequential assignment procedure is outlined, based on two-dimensional NOE ( NOESY ) and two-dimensional J-correlated spectroscopy ( COSY ), for assigning the nonexchangeable proton resonances in NMR spectra of oligonucleotides. As presented here the method is generally applicable to right-handed helical oligonucleotides of intermediate size. We applied it to a lac operator DNA fragment consisting of d( TGAGCGG ) and d( CCGCTCA ) and obtained complete assignments for the adenine H8, guanine H8, cytosine H6 and H5, thymine H6 and 5-methyl, and the deoxyribose H1', H2', H2", H3', and H4' resonances, as well as some H5', H5" (pairwise) assignments. These assignments are required for the analysis of two-dimensional NOE and J-coupling data in terms of the solution structure of oligonucleotides.
本文概述了一种基于二维核Overhauser效应（NOESY）和二维J-相关光谱（COSY）的顺序归属程序，用于确定寡核苷酸核磁共振谱中不可交换质子的共振峰。本文所介绍的方法通常适用于中等大小的右手螺旋寡核苷酸。我们将其应用于由d（TGAGCGG）和d（CCGCTCA）组成的乳糖操纵子DNA片段，完成了腺嘌呤H8、鸟嘌呤H8、胞嘧啶H6和H5、胸腺嘧啶H6和5-甲基以及脱氧核糖H1'、H2'、H2"、H3'和H4'共振峰的归属，以及一些H5'、H5"（成对）的归属。这些归属对于根据寡核苷酸的溶液结构分析二维NOE和J-耦合数据是必需的。
