水溶性碳二亚胺对腺苷-5'-多磷酸盐的作用。
The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates.
作者信息
Ng K E, Orgel L E
出版信息
Nucleic Acids Res. 1987 Apr 24;15(8):3573-80. doi: 10.1093/nar/15.8.3573.
Abstract
When aqueous solutions of adenosine-5'-mono-, di-, or triphosphates are treated with a water soluble carbodiimide the major product is the expected diadenosine-5'-5'-polyphosphate. The yields of these pyrophosphates are greatly increased in the presence of the Mg2+ ion. Adenosine-5'-tetraphosphate behaves differently. The major product is adenosine-5'-monophosphate. We believe that this hydrolysis occurs via a cyclic trimetaphosphate intermediate.
摘要
当腺苷 - 5'-单磷酸、二磷酸或三磷酸的水溶液用一种水溶性碳二亚胺处理时，主要产物是预期的5'-5'-二腺苷多磷酸。在Mg2 +离子存在下，这些焦磷酸盐的产率大大提高。腺苷 - 5'-四磷酸的行为不同。主要产物是腺苷 - 5'-单磷酸。我们认为这种水解是通过环状三偏磷酸中间体发生的。
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