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连接中间体及相关多核苷酸焦磷酸的制备。

Preparation of ligation intermediates and related polynucleotide pyrophosphates.

作者信息

Chu B C, Orgel L E

出版信息

Biochim Biophys Acta. 1984 May 15;782(1):103-5. doi: 10.1016/0167-4781(84)90111-8.

DOI:10.1016/0167-4781(84)90111-8
PMID:6326832
Abstract

Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.

摘要

未受保护的寡核苷酸和以无阻碍的5'-磷酸基团结尾的寡脱氧核苷酸在水溶液中与核苷5'-磷酰咪唑反应，以至少70%的产率生成“封端”的焦磷酸盐。如果在反应中使用腺苷5'-磷酰咪唑作为底物，则会得到连接酶中间体作为产物。

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2
N-methylimidazolium chloride-catalyzed pyrophosphate formation: application to the synthesis of Lipid I and NDP-sugar donors.N-甲基咪唑氯催化焦磷酸形成：在脂质 I 和 NDP-糖供体合成中的应用。
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3
Montmorillonite catalysis of 30-50 mer oligonucleotides: laboratory demonstration of potential steps in the origin of the RNA world.
蒙脱石对30 - 50聚体寡核苷酸的催化作用：RNA世界起源潜在步骤的实验室论证
Orig Life Evol Biosph. 2002 Aug;32(4):311-32. doi: 10.1023/a:1020543312109.
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Oligomerization of activated D- and L-guanosine mononucleotides on templates containing D- and L-deoxycytidylate residues.活化的D-和L-鸟苷单核苷酸在含有D-和L-脱氧胞苷酸残基的模板上的寡聚化。
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13448-52. doi: 10.1073/pnas.95.23.13448.
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Information transfer from peptide nucleic acids to RNA by template-directed syntheses.通过模板导向合成实现肽核酸向RNA的信息传递。
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The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates.水溶性碳二亚胺对腺苷-5'-多磷酸盐的作用。
Nucleic Acids Res. 1987 Apr 24;15(8):3573-80. doi: 10.1093/nar/15.8.3573.
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Reaction on alternating GC oligonucleotide templates.
J Mol Evol. 1989 Aug;29(2):101-7. doi: 10.1007/BF02100109.
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Mineral catalysis of the formation of dimers of 5'-AMP in aqueous solution: the possible role of montmorillonite clays in the prebiotic synthesis of RNA.水溶液中5'-AMP二聚体形成的矿物催化作用：蒙脱石粘土在RNA益生元合成中的可能作用。
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Selective cleavage of pyrophosphate linkages.焦磷酸键的选择性裂解
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