连接中间体及相关多核苷酸焦磷酸的制备。
Preparation of ligation intermediates and related polynucleotide pyrophosphates.
作者信息
Chu B C, Orgel L E
出版信息
Biochim Biophys Acta. 1984 May 15;782(1):103-5. doi: 10.1016/0167-4781(84)90111-8.
PMID:6326832
Abstract
Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.
摘要
未受保护的寡核苷酸和以无阻碍的5'-磷酸基团结尾的寡脱氧核苷酸在水溶液中与核苷5'-磷酰咪唑反应，以至少70%的产率生成“封端”的焦磷酸盐。如果在反应中使用腺苷5'-磷酰咪唑作为底物，则会得到连接酶中间体作为产物。
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