人血小板中的腺苷（5'）寡磷酸 -（5'）鸟苷和鸟苷（5'）寡磷酸 -（5'）鸟苷
Adenosine(5') oligophospho-(5') guanosines and guanosine(5') oligophospho-(5') guanosines in human platelets.
作者信息
Schlüter H, Grobeta I, Bachmann J, Kaufmann R, van der Giet M, Tepel M, Nofer J R, Assmann G, Karas M, Jankowski J, Zidek W
机构信息
Med. Klinik I, Univ.-Klinik Marienhospital, University of Bochum, Hölkeskampring 40, 44625 Herne, Germany.
出版信息
J Clin Invest. 1998 Feb 1;101(3):682-8. doi: 10.1172/JCI119882.
Abstract
We isolated and identified nucleoside(5') oligophospho-(5') nucleosides containing adenosine and guanosine (ApnG; n = 3-6) as well as diguanosine polyphosphates (GpnG; n = 3-6) in human platelets. For identification, UV spectrometry, matrix-assisted laser desorption/ionization, postsource decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic cleavage experiments were used. The adenosine(5') oligophospho-(5') guanosines act as vasoconstrictors and growth factors. The diguanosine polyphosphates are potent modulators of growth in vascular smooth muscle cells, but do not affect vascular tone.
摘要
我们在人血小板中分离并鉴定出含有腺苷和鸟苷的核苷（5'）寡磷酸 -（5'）核苷（ApnG；n = 3 - 6）以及双鸟苷多磷酸盐（GpnG；n = 3 - 6）。为进行鉴定，使用了紫外光谱法、基质辅助激光解吸/电离、源后衰变基质辅助激光解吸/电离质谱法以及酶切实验。腺苷（5'）寡磷酸 -（5'）鸟苷充当血管收缩剂和生长因子。双鸟苷多磷酸盐是血管平滑肌细胞生长的有效调节剂，但不影响血管张力。
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