Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China.
Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China.
Chemosphere. 2019 Jan;215:454-460. doi: 10.1016/j.chemosphere.2018.10.070. Epub 2018 Oct 13.
We established the mouse model of fluoride (68 mg F ion/L deionized water) exposure for 90 days, 120 days and 150 days, and applied diverse methods as behavioral models of anxiety and depression, and analyzed the levels of the anxiety- and depression-like related genes like BDNF1, BDNF4, 5-HT1A, VGLUT, GAD67, and VGAT in the mouse hippocampus. In the mice exposed to NaF for 120 days, compared to the control group, chalky opacity was observed on the enamel of teeth; the results of anxiety-like behavior, like elevated zero maze, light/dark exploration test, novel object recognition test and emergence test were significantly altered, however in the mice exposed for 150 days, only the elevated zero maze and emergence test were significantly altered. Also, the results of depression-like behavior were significantly altered in the 120 days treated mice. Exposure to NaF for 120 days significantly decreased the mRNA expression levels of the BDNF4 with a concomitant increase in the 5-HT1A compared to the control mice. Especially the mRNA expression levels of GAD67 and VGAT were significantly decreased in all the three NaF treated groups. However, no significant changes were observed in the mRNA expression levels of the VGLUT compared to the control mice. In summary, we speculated that fluoride exposure had adverse effects on nervous system, inducing an imbalance between excitation and inhibition, which resulted in abnormal behavior and depression.
我们建立了氟（68mgF 离子/ L 去离子水）暴露 90 天、120 天和 150 天的小鼠模型，并应用多种方法作为焦虑和抑郁的行为模型，分析了小鼠海马中与焦虑和抑郁相关的基因（如 BDNF1、BDNF4、5-HT1A、VGLUT、GAD67 和 VGAT）的水平。在暴露于 NaF 的小鼠中，与对照组相比，牙齿的牙釉质出现白垩状不透明；类似焦虑的行为，如高架十字迷宫、明暗探索试验、新物体识别试验和出现试验的结果显著改变，但在暴露 150 天的小鼠中，仅高架十字迷宫和出现试验的结果显著改变。此外，120 天处理组的类似抑郁的行为结果也显著改变。与对照组相比，暴露于 NaF 120 天显著降低了 BDNF4 的 mRNA 表达水平，同时 5-HT1A 增加。特别是在所有三个 NaF 处理组中，GAD67 和 VGAT 的 mRNA 表达水平显著降低。然而，与对照组相比，VGLUT 的 mRNA 表达水平没有观察到显著变化。综上所述，我们推测氟暴露对神经系统有不良影响，导致兴奋和抑制之间失衡，从而导致异常行为和抑郁。
