College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
College of Food Science and Engineering, Shandong Agriculture and Engineering University, Jinan 250100, Shandong, China.
Molecules. 2018 Oct 17;23(10):2667. doi: 10.3390/molecules23102667.
Probiotic bacteria is able to metabolize polyphenols and produce functional compounds. In this study, we investigated the ability of probiotic bacteria including , bifidobacteria and strains to increase the antioxidant capacity of polyphenols from lotus seed epicarp (PLSE) at full ripening stage. The results showed that the six selected strains of probiotic bacteria grew well in De Man, Rogosa and Sharpe (MRS) broth with PLSE, and their resistant extent to PLSE varied from strain to strain. The metabolized PLSE was found to have good antioxidant properties on 3-ethylbenzothiazoline-6-sulfonic acid (ABTS⁺) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals in vitro. Five polyphenol compounds-chlorogenic acid, caffeic acid, catechin, epicatechin and hyperoside-were suggested as the major bioactive metabolism for the antiradical activity of PLSE metabolized by DSM20016, M74 and ATCC 15701. Moreover, DSM20016 and M74 were found to have a high PLSE bioconversion rate. Our results suggested that both DSM20016 and M74 might have excellent potential for the bioconversion of PLSE to increase its antiradical activity.
益生菌能够代谢多酚并产生功能性化合物。在这项研究中，我们研究了包括双歧杆菌和乳杆菌在内的益生菌菌株增加完全成熟阶段莲子皮中多酚的抗氧化能力的能力。结果表明，在添加莲子皮的德氏乳杆菌培养基（MRS）中，六种选定的益生菌菌株生长良好，其对莲子皮的抗性程度因菌株而异。体外实验发现，代谢后的莲子皮对 3-乙基苯并噻唑啉-6-磺酸（ABTS⁺）和 1,1-二苯基-2-苦基肼（DPPH）自由基具有良好的抗氧化性能。推测绿原酸、咖啡酸、儿茶素、表儿茶素和金丝桃苷五种多酚化合物是 DSM20016、M74 和 ATCC 15701 代谢莲子皮的抗自由基活性的主要生物活性代谢产物。此外，发现 DSM20016 和 M74 具有较高的莲子皮生物转化率。我们的研究结果表明，DSM20016 和 M74 都可能具有将莲子皮转化为增加其抗氧化活性的优异潜力。
