University of Agronomic Science and Veterinary Medicine, Faculty of Biotechnology, 59 Marasti Blvd, 1 District, 011464 Bucharest, Romania.
Centre of Bioanalysis, National Institute for Biological Sciences, 296 Spl. Independentei, 060031 Bucharest, Romania.
Molecules. 2018 Aug 23;23(9):2128. doi: 10.3390/molecules23092128.
This study presents the effect of two new products based on atomized extracts from edible wild mushrooms (RoBioMush1, RoBioMush2) on the microbiota of three target groups: clinically healthy (NG) individuals, individuals with nutritional disorders (ND), and individuals with cardiovascular diseases (CVD). The microbiota fingerprints were determined by quantitative polymerase chain reaction (qPCR). Modulations in the simulated microbiome were established and correlated with the presence of phenolic compounds released in the in vitro environment (a three-stage culture system GIS2 simulator, www.gissystems.ro). The high metabolizing capacity of NG and CVD correlated positively with the rest of the biological activities expressed in vitro. ND microbiota consumed a wide spectrum of monosaccharides from the products. Xylose was present in large quantities in the descending segment (minimum: 175 μg/mL for ND). The primary conclusion was that the microbiological ecosystem was modulated, as proven by the presence of specific biomarkers (e.g., ammonium levels and fingerprints of short-chain fatty acids⁻SCFAs), which stimulate the organism's health status and were correlated with the restoration of a normal microbiota fingerprint.
本研究展示了两种基于可食用野生蘑菇雾化提取物的新产品（RoBioMush1、RoBioMush2）对三个目标群体的微生物群的影响：临床健康个体（NG）、营养紊乱个体（ND）和心血管疾病个体（CVD）。通过定量聚合酶链反应（qPCR）确定微生物指纹图谱。在体外环境中（三阶段培养系统 GIS2 模拟器，www.gissystems.ro），建立了模拟微生物组的调制，并与释放的酚类化合物的存在相关联。NG 和 CVD 的高代谢能力与体外表达的其余生物活性呈正相关。ND 微生物群从产品中消耗了广泛的单糖。木糖在下降段大量存在（最小值：ND 为 175 μg/mL）。主要结论是微生物生态系统被调制，这可以通过存在特定的生物标志物（例如，铵水平和短链脂肪酸指纹⁻SCFAs）来证明，这些标志物刺激了生物体的健康状况，并与恢复正常微生物群指纹相关联。
