Wang Moxi, Feng Li, Fan Xiaowei, Li Dongmei, Qu Wenqi, Jiang Shuxian, Li Shaoxiu
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
School of Civil and Transportation Engineering, Guangdong University of Technology, No. 100, Waihuan Xi Road, Higher Education Mega Center, Panyu District, Guangzhou 510006, Guangdong, China.
Materials (Basel). 2018 Oct 17;11(10):2009. doi: 10.3390/ma11102009.
In this study, a series of chitosan-based quaternary ammonium graft flocculants, namely chitosan--poly(acrylamide and methacryloyl ethyl trimethyl ammonium chloride) [CTS--P(AM-DMC)], was successfully synthesized by plasma initiation, and the as-prepared [CTS--P(AM-DMC)] had both flocculation and sterilization functions. Various characterization techniques were used to study the structure and physicochemical properties of the chitosan-based flocculants. ¹H nuclear magnetic resonance spectroscopy (¹H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and thermogravimetric analysis/differential scanning calorimetry (TG/DSC) confirmed the successful synthesis of CTS--P(AM-DMC). Scanning electron microscopy (SEM) analysis exhibited that CTS--P(AM-DMC) contained a smooth convex and porous structure with an enormous surface area. CTS--P(AM-DMC) was then used to flocculate the simulated wastewater of the kaolin suspension and the suspension. Besides external factors, such as the dosage of flocculant and pH, the effect of the internal factor graft ratio was also evaluated. The experimental results showed that CTS--P(AM-DMC) also revealed a strong sterilization effect, aside from the excellent flocculation effect. Moreover, the sterilization mechanism was investigated through a series of conductivity measurements and the analysis of fluorescence-based cell live/dead tests. The results indicated that CTS--P(AM-DMC) destroyed the cell membrane of through its grafted quaternary ammonium salt, thereby exhibiting sterilization property.
在本研究中，通过等离子体引发成功合成了一系列基于壳聚糖的季铵接枝絮凝剂，即壳聚糖-聚（丙烯酰胺和甲基丙烯酰氧乙基三甲基氯化铵）[CTS-P(AM-DMC)]，所制备的[CTS-P(AM-DMC)]兼具絮凝和杀菌功能。采用各种表征技术研究了基于壳聚糖的絮凝剂的结构和理化性质。¹H核磁共振光谱（¹H NMR）、傅里叶变换红外光谱（FTIR）、X射线衍射光谱（XRD）和热重分析/差示扫描量热法（TG/DSC）证实了CTS-P(AM-DMC)的成功合成。扫描电子显微镜（SEM）分析表明，CTS-P(AM-DMC)具有光滑的凸起和多孔结构，表面积巨大。然后将CTS-P(AM-DMC)用于絮凝高岭土悬浮液和该悬浮液的模拟废水。除了絮凝剂用量和pH等外部因素外，还评估了内部因素接枝率的影响。实验结果表明，CTS-P(AM-DMC)除了具有优异的絮凝效果外，还具有很强的杀菌效果。此外，通过一系列电导率测量和基于荧光的细胞死活测试分析研究了杀菌机制。结果表明，CTS-P(AM-DMC)通过其接枝的季铵盐破坏了该菌的细胞膜，从而表现出杀菌性能。
