Nassar B A, Manku M S, Huang Y S, Jenkins D K, Horrobin D F
Prostaglandins Leukot Med. 1987 Mar;26(3):253-63. doi: 10.1016/0262-1746(87)90035-7.
The interactions of n-6 and n-3 fatty acids on prostaglandin metabolism in the isolated rat mesenteric vessels were studied. Sprague-Dawley rats (200-220 g) were fed for two weeks a fat-free semi-synthetic diet supplemented with 10% by weight of different combinations of Evening Primrose Oil (Efamol), a rich source of linoleic acid (LA) and gamma-linolenic acid (GLA), the immediate precursor of dihomo-gamma-linolenic acid (DGLA), and Polepa (POL), a marine oil rich in eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. The combinations of supplement were as follows: 9% Efamol - 1% POL, 8% Efamol - 2% POL, 7% Efamol - 3% POL, 6% Efamol - 4% POL, 5% Efamol - 5% POL. The outflow of thromboxane (TxB2), prostacyclin (6-keto-PGF1 alpha), PGE2, and PGE1 was decreased in relation to the proportion of marine oil in the diet, except for the group which received 8% Efamol - 2% POL, and which showed an increase in 6-keto-PGF1 alpha, PGE2, and PGE1. The decrease in TxB2 was much greater than those of 6-keto-PGF1 alpha or PGE2, while PGE1 followed the same pattern as prostacyclin and PGE2. These results suggest that n-3 fatty acids, at high concentrations, inhibits conversion of both DGLA and AA to eicosanoids. Low concentrations of fish oil may, in contrast, increase formation of desirable 1 and 2 series eicosanoids.
研究了n-6和n-3脂肪酸对离体大鼠肠系膜血管中前列腺素代谢的相互作用。给体重200 - 220克的斯普拉格-道利大鼠喂食两周无脂半合成饮食，该饮食补充了按重量计10%的不同组合的月见草油（Efamol）和Polepa（POL），月见草油是亚油酸（LA）和γ-亚麻酸（GLA）的丰富来源，γ-亚麻酸是二高-γ-亚麻酸（DGLA）的直接前体，Polepa是富含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的鱼油。补充剂的组合如下：9%月见草油 - 1% POL、8%月见草油 - 2% POL、7%月见草油 - 3% POL、6%月见草油 - 4% POL、5%月见草油 - 5% POL。血栓素（TxB2）、前列环素（6-酮-PGF1α）、PGE2和PGE1的流出量随饮食中海鱼油的比例而降低，但接受8%月见草油 - 2% POL的组除外，该组6-酮-PGF1α、PGE2和PGE1有所增加。TxB2的降低幅度远大于6-酮-PGF1α或PGE2，而PGE1与前列环素和PGE2的模式相同。这些结果表明，高浓度的n-3脂肪酸会抑制DGLA和花生四烯酸（AA）向类二十烷酸的转化。相比之下，低浓度的鱼油可能会增加所需的1和2系列类二十烷酸的形成。
