Huang Y S, Nassar B A, Horrobin D F
Efamol Research Institute, Kentville, Nova Scotia, Canada.
Prostaglandins Leukot Essent Fatty Acids. 1989 Feb;35(2):73-9. doi: 10.1016/0952-3278(89)90168-3.
The effects of dietary n-6 polyunsaturated fatty acids and replacement with saturated fat or fish oil on the prostaglandin outflow from perfused mesenteric vasculature in rats were studied. Seventy-two weanling male rats were fed ad libitum a semi-synthetic diet supplemented with 10% by weight of oil, composed wholly of n-6 fatty acid-rich evening primrose oil, or replaced partly or completely (25, 50, 75 or 100%) by n-6 fatty acid-deficient fish oil or hydrogenated coconut oil for 8 weeks. The outflows of 6-keto-PGF1 alpha, thromboxane B2, and prostaglandin E from the perfused mesenteric vasculature were measured at 60 min-time point after starting the perfusion. In general, the release of prostanoids from the mesenteric vasculature was significantly reduced in rats fed a diet in which evening primrose oil was partly or completely replaced by either hydrogenated coconut or fish oil. This was probably due to the insufficient conversion of linoleic acid to arachidonic acid. The extent of reduction was greater in fish oil-fed than in hydrogenated coconut oil-fed rats, while the levels of arachidonic acid in aortic phospholipids were similar between these two groups. This result implies that the greater reduction of prostaglandin synthesis in rats fed fish oil was due to the inhibitory effect of eicosapentaenoic and docosahexaenoic acids in fish oil on the conversion of arachidonate to eicosanoids.
研究了膳食中n-6多不饱和脂肪酸以及用饱和脂肪或鱼油替代对大鼠灌注肠系膜血管中前列腺素流出的影响。72只断乳雄性大鼠随意进食一种半合成饮食，该饮食补充了10%（重量）的油，油完全由富含n-6脂肪酸的月见草油组成，或者部分或完全（25%、50%、75%或100%）被缺乏n-6脂肪酸的鱼油或氢化椰子油替代，持续8周。在开始灌注后60分钟时间点测量灌注肠系膜血管中6-酮-PGF1α、血栓素B2和前列腺素E的流出量。一般来说，在食用月见草油部分或完全被氢化椰子油或鱼油替代饮食的大鼠中，肠系膜血管中前列腺素类的释放显著减少。这可能是由于亚油酸向花生四烯酸的转化不足。鱼油喂养的大鼠中减少程度大于氢化椰子油喂养的大鼠，而这两组大鼠主动脉磷脂中花生四烯酸的水平相似。这一结果表明，鱼油喂养的大鼠中前列腺素合成减少幅度更大是由于鱼油中的二十碳五烯酸和二十二碳六烯酸对花生四烯酸向类二十烷酸转化的抑制作用。
