Nassar B A, Huang Y S, Manku M S, Das U N, Morse N, Horrobin D F
Lipids. 1986 Oct;21(10):652-6. doi: 10.1007/BF02537216.
The interrelations between linoleic acid (LA) metabolites and fish oil fatty acids were studied. Sprague-Dawley rats (200-220 g) were fed a fat-free semisynthetic diet supplemented with 10% (by weight) of different combinations of evening primrose oil (EPO), a rich source of LA and gamma-linolenic acid, and polepa (POL), a marine oil rich in eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. The combinations of supplement were as follows: 9% EPO-1% POL, 8% EPO-2% POL, 7% EPO-3% POL, 6% EPO-4% POL and 5% EPO-5% POL. After two weeks on the respective diets, the animals were killed, and the fatty acid compositions of liver and plasma phospholipids were examined. The results showed that animals fed higher proportions of POL consistently contained higher levels of dihomo-gamma-linolenic acid (DGLA) (p less than 0.05), a metabolite of LA and GLA, and lower levels of arachidonic acid (AA) (p less than 0.01), a metabolite of DGLA through delta-5-desaturation. Thus, an inverse relationship between AA/DGLA ratio and EPA levels was found to exist (r = -0.765 in plasma and -0.792 in liver). However, there was no such relationship between AA/DGLA ratio and DHA levels. This result suggested that EPA but not DHA in fish oil exerts an inhibitory effect on the conversion of DGLA to AA.
研究了亚油酸（LA）代谢产物与鱼油脂肪酸之间的相互关系。给体重200 - 220克的斯普拉格-道利大鼠喂食无脂半合成饮食，并添加10%（按重量计）不同组合的月见草油（EPO）和极地鱼油（POL），月见草油是LA和γ-亚麻酸的丰富来源，极地鱼油是富含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的海产油。补充剂的组合如下：9% EPO - 1% POL、8% EPO - 2% POL、7% EPO - 3% POL、6% EPO - 4% POL和5% EPO - 5% POL。在各自饮食喂养两周后，处死动物，检测肝脏和血浆磷脂的脂肪酸组成。结果表明，喂食较高比例POL的动物体内，LA和GLA的代谢产物二高-γ-亚麻酸（DGLA）水平持续较高（p < 0.05），而通过δ-5去饱和作用由DGLA生成的花生四烯酸（AA）水平较低（p < 0.01）。因此，发现AA/DGLA比值与EPA水平之间存在负相关（血浆中r = -0.765，肝脏中r = -0.792）。然而，AA/DGLA比值与DHA水平之间不存在这种关系。该结果表明，鱼油中的EPA而非DHA对DGLA向AA的转化具有抑制作用。
