Department of Applied Bioscience, Kanagawa Institute of Technology, Kanagawa, Japan.
Department of Applied Bioscience, Kanagawa Institute of Technology, Kanagawa, Japan.
Biochem Biophys Res Commun. 2018 Nov 17;506(1):53-59. doi: 10.1016/j.bbrc.2018.10.021. Epub 2018 Oct 15.
Activation of thermogenic adipocytes (brown and beige) has been considered an attractive target for weight loss and treatment of metabolic disease. Peroxisome proliferator-activated receptor γ co-activator-1 α (PGC1-α) is a master regulator of thermogenic gene expression in thermogenic adipocytes. We previously reported that α-tocopherol upregulated PGC-1α gene expression and promoted thermogenic adipocyte differentiation in mammalian adipocytes. In this study, we investigated the effects of the vitamin E analogs (α-, γ- and δ-tocopherol) on PGC-1α and uncoupling protein 1 (UCP1) gene expression in 3T3-L1 cells. The expression of PGC-1α and UCP1 increased significantly with the addition of δ-tocopherol. In δ-tocopherol-treated cells, nuclear translocation of PGC-1α increased, as did p38 mitogen-activated protein kinase (MAPK) expression and phosphorylation. Our results suggest that p38 MAPK activation by δ-tocopherol contributes to PGC-1α activation and UCP1 induction.
脂肪细胞（棕色和米色）的产热激活一直被认为是减肥和治疗代谢疾病的一个有吸引力的靶点。过氧化物酶体增殖物激活受体 γ 共激活因子 1α（PGC1-α）是产热脂肪细胞产热基因表达的主要调节因子。我们之前曾报道，α-生育酚上调 PGC-1α 基因表达，并促进哺乳动物脂肪细胞的产热脂肪细胞分化。在这项研究中，我们研究了维生素 E 类似物（α-、γ-和 δ-生育酚）对 3T3-L1 细胞中 PGC-1α 和解偶联蛋白 1（UCP1）基因表达的影响。随着 δ-生育酚的添加，PGC-1α 和 UCP1 的表达显著增加。在 δ-生育酚处理的细胞中，PGC-1α 的核转位增加，p38 有丝分裂原激活蛋白激酶（MAPK）的表达和磷酸化也增加。我们的结果表明，δ-生育酚通过 p38 MAPK 激活有助于 PGC-1α 的激活和 UCP1 的诱导。
