Coughlin Adam M, Nagelkirk Paul R, Cooper Jamie A, Paton Chad M, Friderici Karen H, Wingerd Byron A, Pivarnik James M, Womack Christopher J
Department of Kinesiology, Saginaw Valley State University, University Center, MI, USA.
School of Kinesiology, Ball State University, Muncie, IN, USA.
Int J Exerc Sci. 2018 Oct 1;11(3):1136-1144. doi: 10.70252/HFSZ9901. eCollection 2018.
The purpose was to determine if the Alu-insertion (I)/deletion (D) polymorphism of the tissue plasminogen activator (tPA) gene influences the tPA response to maximal exercise. Fifty male subjects (age = 23.6 ± 4.7 yrs) completed a maximal treadmill exercise test. Blood samples were drawn before and immediately after exercise for determination of plasma tPA antigen and activity. Isolated DNA was amplified via polymerase chain reaction, electrophoresed, and visually amplified to determine tPA genotype. Subjects were classified as possessing the D allele (D) (n = 28) or being homozygous for the I allele (I) (n = 22). Differences in tPA antigen and activity were assessed using a two-factor (genotype and time) repeated measures analysis of variance. There were significant main effects for time for tPA antigen and activity (p < 0.05), but no main effect for genotype. Furthermore, there was no genotype x time interaction due to a similar increase in tPA antigen in the D group (pre-exercise = 5.83 + 2.39 ng/ml, post-exercise = 21.88 + 7.38 ng/ml) and the I group (pre-exercise = 5.61 + 2.82 ng/ml, post-exercise = 19.05 + 7.67 ng/ml) and a similar increase in tPA activity in the D group (pre-exercise = 0.39 ± 0.19 IU/ml, post-exercise = 9.73 ± 4.22 IU/ml) and I group (pre-exercise = 0.45 ± 0.29 IU/ml, post-exercise = 9.76 ± 5.50 IU/ml). The I/D polymorphism of the tPA gene does not influence the tPA antigen nor tPA activity responses to maximal exercise in healthy, young, sedentary males.
目的是确定组织型纤溶酶原激活剂（tPA）基因的Alu插入（I）/缺失（D）多态性是否会影响tPA对最大运动的反应。五十名男性受试者（年龄 = 23.6 ± 4.7岁）完成了一次最大强度跑步机运动测试。在运动前和运动后立即采集血样，以测定血浆tPA抗原和活性。通过聚合酶链反应扩增分离的DNA，进行电泳，并通过肉眼观察扩增结果以确定tPA基因型。受试者被分为具有D等位基因（D）（n = 28）或为I等位基因纯合子（I）（n = 22）。使用双因素（基因型和时间）重复测量方差分析评估tPA抗原和活性的差异。tPA抗原和活性在时间上有显著的主效应（p < 0.05），但基因型没有主效应。此外，由于D组（运动前 = 5.83 + 2.39 ng/ml，运动后 = 21.88 + 7.38 ng/ml）和I组（运动前 = 5.61 + 2.82 ng/ml，运动后 = 19.05 + 7.67 ng/ml）中tPA抗原的类似增加，以及D组（运动前 = 0.39 ± 0.19 IU/ml，运动后 = 9.73 ± 4.22 IU/ml）和I组（运动前 = 0.45 ± 0.29 IU/ml，运动后 = 9.76 ± 5.50 IU/ml）中tPA活性的类似增加，不存在基因型×时间交互作用。tPA基因的I/D多态性不影响健康、年轻、久坐男性对最大运动的tPA抗原和tPA活性反应。
