Reĭtarova T E, Rozanov V A, Totskiĭ V N
Ukr Biokhim Zh (1978). 1987 Mar-Apr;59(2):87-90.
The [14C]GABA uptake by slices (0.3 mm thick) of Wistar rat brain cortex was studied for its dependence on the GABA concentration in the medium, time of incubation and the presence of Ca2+. This process is characterized by the absence of saturation; the uptake by slices increases sharply when the concentration of exogenous [14C]GABA reaches 200 microM. Bicucullin (10(-4) M), an antagonist of GABA, inhibits the accumulation of GABA in the concentration of 0.2 microM by 60%, that evidences for a considerable contribution of the receptor binding to this process. The [14C]GABA uptake when Ca2+ is absent in the incubation medium and when its concentration is 10(-3) M is practically the same and comparatively low concentrations of Ca2+ (10(-6)-10(-4] decrease the GABA uptake.
研究了Wistar大鼠大脑皮层切片（0.3毫米厚）对[14C]GABA的摄取，考察其对培养基中GABA浓度、孵育时间和Ca2+存在与否的依赖性。该过程的特点是不存在饱和现象；当外源性[14C]GABA浓度达到200微摩尔时，切片摄取量急剧增加。GABA拮抗剂荷包牡丹碱（10^-4 M）在0.2微摩尔浓度下可抑制GABA积累60%，这表明受体结合对此过程有相当大的贡献。孵育培养基中不存在Ca2+以及其浓度为10^-3 M时，[14C]GABA摄取量实际上相同且相对较低，较低浓度的Ca2+（10^-6 - 10^-4）会降低GABA摄取量。
