环状 RNA circ-NT5C2 可作为骨肉瘤预后的潜在新型生物标志物。

Circular RNA circ-NT5C2 acts as a potential novel biomarker for prognosis of osteosarcoma.

机构信息

Qingdao University Medical College, Qingdao, Shandong, China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Oct;22(19):6239-6244. doi: 10.26355/eurrev_201810_16030.

DOI:10.26355/eurrev_201810_16030

PMID:30338784

Abstract

OBJECTIVE

Recent evidence suggests that circular RNAs (circRNAs) play important roles in multiple diseases, including cancer. Circ-NT5C2 was reported to be up-regulated in osteosarcoma. However, the clinical significance of circ-NT5C2 remains largely unclear. The aim of the current study was to investigate the value of circ-NT5C2 for the prognosis of patients with osteosarcoma.

PATIENTS AND METHODS

the expression of circ-NT5C2 in osteosarcoma tissues and corresponding normal tissues were explored by quantitative real-time polymerase chain reaction (qRT-PCR) experiments. The association of circ-NT5C2 expression with clinicopathological factors or the prognosis of osteosarcoma patients was also analyzed. Kaplan-Meier survival analysis was performed to analyze the association of circ-NT5C2 expression with overall survival (OS) and disease-free survival (DFS) of patients. Univariate and multivariate Cox-regression analyses were used to identify risk factors for poor prognosis.

RESULTS

Our data showed a significant increase of circ-NT5C2 expression in osteosarcoma tissues compared with adjacent normal bone tissues (p < 0.01). In addition, we found that the level of circ-NT5C2 in osteosarcoma was strongly correlated with clinical stage (p = 0.006) and distant metastasis (p = 0.001). Importantly, patients with high expression of circ-NT5C2 had a shorter OS (p = 0.006) and DFS (p = 0.001) than those with low expression of circ-NT5C2. Finally, Cox regression analyses showed that high circ-NT5C2 expression might be an independent prognostic parameter to predict poor prognosis.

CONCLUSIONS

Our findings indicated that circ-NT5C2 is significantly up-regulated in osteosarcoma tissues. Circ-NT5C2 may represent a new marker of prognosis in osteosarcoma.

摘要

目的

最近的证据表明，环状 RNA（circRNA）在包括癌症在内的多种疾病中发挥着重要作用。Circ-NT5C2 在骨肉瘤中被报道上调。然而，circ-NT5C2 的临床意义在很大程度上仍不清楚。本研究旨在探讨 circ-NT5C2 对骨肉瘤患者预后的价值。

方法

通过定量实时聚合酶链反应（qRT-PCR）实验研究了骨肉瘤组织和相应正常组织中 circ-NT5C2 的表达。还分析了 circ-NT5C2 表达与骨肉瘤患者临床病理因素或预后的关系。Kaplan-Meier 生存分析用于分析 circ-NT5C2 表达与患者总生存期（OS）和无病生存期（DFS）的关系。单因素和多因素 Cox 回归分析用于确定不良预后的危险因素。

结果

我们的数据显示，骨肉瘤组织中 circ-NT5C2 的表达明显高于相邻正常骨组织（p < 0.01）。此外，我们发现骨肉瘤中 circ-NT5C2 的水平与临床分期（p = 0.006）和远处转移（p = 0.001）密切相关。重要的是，circ-NT5C2 高表达的患者 OS（p = 0.006）和 DFS（p = 0.001）均短于 circ-NT5C2 低表达的患者。最后，Cox 回归分析表明，circ-NT5C2 高表达可能是预测不良预后的独立预后参数。