Department of Gynecology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Eur Rev Med Pharmacol Sci. 2018 Oct;22(19):6260-6267. doi: 10.26355/eurrev_201810_16033.
We investigate whether microRNA-200a could regulate proliferation and invasion of ovarian cancer cells, thereby participating in the occurrence and development of ovarian cancer. We also explore the specific mechanism of microRNA-200a in regulating ovarian cancer.
Expression level of microRNA-200a in ovarian cancer tissues and paracancerous tissues were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-200a on proliferation and invasion of ovarian cancer cells were examined by Cell counting kit-8 (CCK-8) and cell invasion assay, respectively. Dual-luciferase reporter gene assay was performed to confirm the binding relationship between microRNA-200a and PTEN (phosphatase and tensin homolog deleted on chromosome ten). The regulatory role of microRNA-200a in PTEN expression was accessed by Western blot. Rescue experiments were conducted to assess whether microRNA-200a regulated proliferation and invasion of ovarian cancer cells by inhibiting PTEN expression.
MicroRNA-200a expression in ovarian cancer tissues was significantly higher than that of paracancerous tissues. Besides, microRNA-200a was also overexpressed in ovarian cancer cell lines than that of normal ovarian cells. Overexpression of microRNA-200a promoted the proliferative and invasive abilities of SKOV3 and OVCAR3 cells. Dual-luciferase reporter gene assay showed that microRNA-200a could directly degrade PTEN. Overexpression of PTEN in SKOV3 and OVCAR3 cells partially reversed the increased cell proliferation and invasion induced by overexpressed microRNA-200a.
Overexpressed microRNA-200a promoted the proliferative and invasive abilities of ovarian cancer cells, which might be related to the targeted regulation of PTEN expression.
研究 microRNA-200a 是否能调控卵巢癌细胞的增殖和侵袭，从而参与卵巢癌的发生发展。还探索了 microRNA-200a 调控卵巢癌的具体机制。
采用实时定量聚合酶链反应（qRT-PCR）检测卵巢癌组织和癌旁组织中 microRNA-200a 的表达水平。分别通过细胞计数试剂盒-8（CCK-8）和细胞侵袭实验检测 microRNA-200a 对卵巢癌细胞增殖和侵袭的调控作用。双荧光素酶报告基因实验证实 microRNA-200a 与 PTEN（染色体 10 上缺失的磷酸酶及张力蛋白同源物）之间的结合关系。通过 Western blot 检测 microRNA-200a 对 PTEN 表达的调控作用。通过 rescue 实验评估 microRNA-200a 是否通过抑制 PTEN 表达来调节卵巢癌细胞的增殖和侵袭。
卵巢癌组织中 microRNA-200a 的表达明显高于癌旁组织。此外，卵巢癌细胞系中 microRNA-200a 的表达也高于正常卵巢细胞。microRNA-200a 的过表达促进了 SKOV3 和 OVCAR3 细胞的增殖和侵袭能力。双荧光素酶报告基因实验表明，microRNA-200a 可直接降解 PTEN。在 SKOV3 和 OVCAR3 细胞中转染过表达的 PTEN 部分逆转了 microRNA-200a 过表达引起的细胞增殖和侵袭增加。
过表达的 microRNA-200a 促进了卵巢癌细胞的增殖和侵袭能力，这可能与靶向调控 PTEN 表达有关。
