Iizumi Seiichiro, Uchida Fumihiko, Nagai Hiroki, Takaoka Shohei, Fukuzawa Satoshi, Kanno Naomi Ishibashi, Yamagata Kenji, Tabuchi Katsuhiko, Yanagawa Toru, Bukawa Hiroki
Department of Oral and Maxillofacial Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
Heliyon. 2021 Sep 30;7(10):e08086. doi: 10.1016/j.heliyon.2021.e08086. eCollection 2021 Oct.
MicroRNAs (miRNAs) play an important role in carcinogenesis and cancer progression. The purpose of this study was to identify miRNAs associated with carcinoma function in OSCC and to investigate the potential role of the specific miRNAs. First, a comprehensive microarray analysis was performed, and miR-142-5p was identified as a candidate miRNA involved in OSCC. miR-142-5p has been reported to show high expression levels in cancer patients and to be involved in tumor growth and metastasis. However, the expression and function of miR-142-5p in oral squamous cell carcinoma (OSCC) are not fully characterized. We evaluated miR-142-5p expression in both OSCC-derived cell lines and primary OSCC tissues and performed functional analysis of miR-142-5p in OSCC-derived cell lines using mimics and inhibitors. miR-142-5p expression was up-regulated in OSCC tissues and OSCC cell lines. Overexpression of miR-142-5p significantly promoted the proliferation and invasion of OSCC cells. Bioinformatics analysis was performed using TargetScan to predict potential target sites that match the seed region of miR-142-5p. Phosphatase and tensin homolog deleted on chromo-some 10 (PTEN) was identified as a potential target and selected for further analysis. PTEN expression levels were down-regulated and AKT expression levels were up-regulated in miR-142-5p-overexpressing cells. We have shown that miR-142-5p targets the PTEN gene and is involved in cancer progression. Our results suggest that miR-142-5p is involved in the progression of OSCC by controlling the phosphatidylinositol 3-kinase (PI3K)/AKT pathway by targeting the PTEN gene. Our findings suggest that miR-142-5p may be a new target for the treatment of OSCC.
微小RNA（miRNA）在癌症发生和发展过程中发挥着重要作用。本研究旨在鉴定与口腔鳞状细胞癌（OSCC）癌功能相关的miRNA，并探讨特定miRNA的潜在作用。首先，进行了全面的微阵列分析，鉴定出miR-142-5p为参与OSCC的候选miRNA。据报道，miR-142-5p在癌症患者中表达水平较高，并参与肿瘤生长和转移。然而，miR-142-5p在口腔鳞状细胞癌（OSCC）中的表达和功能尚未完全明确。我们评估了miR-142-5p在OSCC来源的细胞系和原发性OSCC组织中的表达，并使用模拟物和抑制剂对OSCC来源的细胞系中的miR-142-5p进行了功能分析。miR-142-5p在OSCC组织和OSCC细胞系中表达上调。miR-142-5p的过表达显著促进了OSCC细胞的增殖和侵袭。使用TargetScan进行生物信息学分析，以预测与miR-142-5p种子区域匹配的潜在靶位点。10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）被鉴定为潜在靶标并选择进行进一步分析。在miR-142-5p过表达的细胞中，PTEN表达水平下调，AKT表达水平上调。我们已经表明，miR-142-5p靶向PTEN基因并参与癌症进展。我们的结果表明，miR-142-5p通过靶向PTEN基因控制磷脂酰肌醇3激酶（PI3K）/AKT途径参与OSCC的进展。我们的研究结果表明，miR-142-5p可能是治疗OSCC的新靶点。
