Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan, 46083, Republic of Korea.
Aquaculture Industry Research Division, East Sea Fisheries Research Institute, Gangneung, 25435, Republic of Korea.
Fish Shellfish Immunol. 2019 Jan;84:485-490. doi: 10.1016/j.fsi.2018.10.041. Epub 2018 Oct 16.
This study investigated the oxidative stress and hemocyte responses of Pacific abalone exposed to various water temperatures (4, 6, 8, and 10 °C) and salinities (26, 30, and 34 psu) for 7 days, to identify their tolerance ranges of temperature and salinity. The survival rate of Pacific abalone ranged from 98.7 to 100% at 8 °C and 10 °C, but dropped to 25-55% at 4 °C at all levels of salinity. The levels of superoxide dismutase and glutathione in the hemolymph were significantly higher at 4 °C and 6 °C than in the controls in all salinity groups, indicating that these temperatures induced greater stress in the Pacific abalone. Total hemocyte count was lowest at 6 °C in the 26 psu group. The percentages of apoptotic and necrotic cells were higher in the 26 psu group than in the other salinity groups, and higher in the 4 °C and 6 °C groups than in the other temperature groups. These results indicate that the lowest tolerance to water temperature and salinity in the Pacific abalone was 8 °C and 30 psu, respectively.
本研究调查了太平洋鲍鱼在不同水温（4、6、8 和 10°C）和盐度（26、30 和 34 psu）下暴露 7 天后的氧化应激和血淋巴细胞反应，以确定其对温度和盐度的耐受范围。在所有盐度水平下，8°C 和 10°C 时太平洋鲍鱼的存活率为 98.7%至 100%，但在 4°C 时降至 25-55%。在所有盐度组中，与对照组相比，4°C 和 6°C 时血淋巴中超氧化物歧化酶和谷胱甘肽的水平显著升高，表明这些温度对太平洋鲍鱼造成了更大的应激。在 26 psu 组中，总血淋巴细胞计数在 6°C 时最低。与其他盐度组相比，26 psu 组的凋亡和坏死细胞比例更高，与其他温度组相比，4°C 和 6°C 组的凋亡和坏死细胞比例更高。这些结果表明，太平洋鲍鱼对水温的最低耐受度为 8°C，对盐度的最低耐受度为 30 psu。
