State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China.
Fish Shellfish Immunol. 2014 Jul;39(1):69-77. doi: 10.1016/j.fsi.2014.04.014. Epub 2014 Apr 30.
Recently, frequent death of cultured abalone drew our attention to the stress tolerance of abalone. Hybridization is an effective way of genetic improvement in aquaculture, which can introduce improved traits to the hybrids. In this study, we challenged the hybrids between Haliotis discus hannai and Haliotis gigantea, and their parents with bacteria (vibrio harveyi, vibrio alginolyticus and vibrio parahemolyticus), then held them at 20 °C and 28 °C, survival rates of the parental populations and hybrid populations were recorded. Then we tested the immune responses and thermal-induced responses of the four populations at different temperatures. Total hemocyte count (THC), respiratory burst, superoxide dismutase activity (SOD), acid phosphatase activity (ACP), alkaline phosphatase activity (AKP), myeloperoxidase activity (MPO), and HSP70 expression were determined on day 1 and day 7 of the temperature exposure. Results showed higher survival rates of the hybrids than their parents against bacteria challenge. For immune parameters, THCs were evaluated at 28 °C, while increased THC was also observed in H. discus hannai ♀ × H. gigantea ♂ (DG) and H. discus hannai ♀ × H. discus hannai ♂ (DD) at 12 °C (day 7); at 28 °C, respiratory burst was activated (day 1 and 7), while SOD activity first rose then fell over 7-days exposure; AKP activity was elevated at 12 °C and 28 °C (day 1), most notably in DG, and an increased level of ACP was observed in DG at 28 °C (day 7); MPO activity was suppressed at 12 °C and 28 °C on day 1, but recovered on day 7. For HSP70, increased HSP70 levels were observed in all populations at 28 °C (day 1), and DD got the lowest HSP70 level after 7-days exposure at 28 °C. Overall, the results suggest that temperature changes could significantly affect the physiological status of abalone, and hybrids may be more resistant to disease and thermal stresses than their parents.
最近，养殖鲍鱼的频繁死亡引起了我们对鲍鱼的抗应激能力的关注。杂交是水产养殖中遗传改良的有效途径，可以将改良的性状引入杂交种中。在这项研究中，我们用细菌（哈维弧菌、溶藻弧菌和副溶血弧菌）挑战了杂交种 Haliotis discus hannai 和 Haliotis gigantea 及其亲本，然后将它们分别置于 20°C 和 28°C 下，记录亲代和杂交种群的存活率。然后，我们在不同温度下测试了这四个种群的免疫反应和热诱导反应。在温度暴露的第 1 天和第 7 天，测定了总血细胞计数（THC）、呼吸爆发、超氧化物歧化酶活性（SOD）、酸性磷酸酶活性（ACP）、碱性磷酸酶活性（AKP）、髓过氧化物酶活性（MPO）和 HSP70 的表达。结果表明，杂交种的存活率高于其亲本对细菌的挑战。对于免疫参数，在 28°C 下评估了 THCs，而在 12°C（第 7 天）下也观察到 H. discus hannai♀×H. gigantea♂（DG）和 H. discus hannai♀×H. discus hannai♂（DD）中 THCs 增加；在 28°C 下，呼吸爆发被激活（第 1 天和第 7 天），而 SOD 活性在 7 天暴露后先升高后下降；AKP 活性在 12°C 和 28°C（第 1 天）升高，在 DG 中最为明显，而在 28°C（第 7 天）时 DG 中 ACP 水平升高；MPO 活性在 12°C 和 28°C 时在第 1 天受到抑制，但在第 7 天恢复。对于 HSP70，在 28°C（第 1 天）时所有种群的 HSP70 水平均升高，而在 28°C 下 7 天暴露后 DD 的 HSP70 水平最低。总体而言，结果表明温度变化会显著影响鲍鱼的生理状态，杂交种可能比其亲本更能抵抗疾病和热应激。
