São Paulo State University (UNESP), School of Dentistry, Department of Basic Sciences, Rua José Bonifácio, 1193, CEP 16015-050, Araçatuba, SP, Brazil; São Paulo State University (UNESP), Institute of Biosciences, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, CEP 18618-689, Botucatu, SP, Brazil; Group for the Research and Study of Laser in Dentistry, São Paulo State University (UNESP), School of Dentistry, Rua José Bonifácio, 1193, CEP 16015-050, Araçatuba, SP, Brazil.
São Paulo State University (UNESP), School of Dentistry, Department of Surgery and Integrated Clinic, Rua José Bonifácio, 1193, CEP 16015-050, Araçatuba, SP, Brazil.
Bone. 2019 Mar;120:101-113. doi: 10.1016/j.bone.2018.10.014. Epub 2018 Oct 16.
This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNFα), interleukin (IL)-1β and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction.
本研究评估了抗菌光动力疗法（aPDT）在具有双膦酸盐相关性颌骨骨坏死（BRONJ）高风险因素的大鼠牙槽修复中的作用。老年大鼠每三天接受 0.45ml 载体（VEH 和 VEH/aPDT）或唑来膦酸（ZOL 和 ZOL/aPDT）。治疗 3 周后，拔除第一下左磨牙。VEH/aPDT 和 ZOL/aPDT 在术后 0、2 和 4 天于拔牙部位行 aPDT。术后 28 天安乐死，通过临床、组织学、组织计量学、组织化学和免疫组织化学分析评估拔牙部位。ZOL 表现出组织修复受损；新生骨组织（NFBT）百分比较低；无活力骨组织（NVBT）百分比较高；成熟胶原纤维较少，肿瘤坏死因子（TNFα）、白细胞介素（IL）-1β 和 IL-6 的免疫标记增加。ZOL/aPDT 显示出与 VEH 相似的拔牙部位的临床和组织学特征、NFBT 百分比和成熟胶原纤维百分比。ZOL/aPDT 中炎症细胞因子的免疫标记物百分比和 NVBT 低于 ZOL。ZOL 和 ZOL/aPDT 中抗酒石酸酸性磷酸酶（TRAP）的免疫标记物较低。牙拔除部位的 aPDT 可改善组织修复过程，并防止拔牙后发生类似 BRONJ 的病变。
