Ogura H, Sato H, Hatano M
Arch Virol. 1987;94(1-2):123-33. doi: 10.1007/BF01313730.
At non-permissive temperature viral specific RNA synthesis was not restricted in rat glioma (C 6) cells infected with HVJ (Sendai virus) wild-type. However, as has previously been shown (J Gen Virol [1984] 65: 639-643), the synthesis of M protein was reduced at non-permissive temperature, in contrast to the L, P, HN, Fo and NP proteins which were synthesized in comparable amounts at permissive and non-permissive temperatures. In this report we show additionally that viral nucleocapsids (NC), which consist of L, P and NP proteins, were formed within the infected cells at both temperatures. Hemagglutinin and neuraminidase activities were also detected in samples incubated at non-permissive temperature. By membrane immunofluorescence and cell-surface immunoprecipitation it was shown that migration of HN and Fo proteins to the cell surface occurred normally at non-permissive temperature. Additionally, the L, P and NP proteins, which were associated with the plasma membrane isolated from the infected cells maintained at permissive temperature, were absent from the membrane of cells incubated at non-permissive temperature. These results suggest that NC and glycoproteins synthesized at non-permissive temperature could not assemble effectively at the plasma membrane because of a lack of M protein. Thus, the host-dependent ts lesion of HVJ in C 6 cells was considered to be mainly in M protein synthesis.
在非允许温度下，感染HVJ（仙台病毒）野生型的大鼠胶质瘤（C6）细胞中病毒特异性RNA合成不受限制。然而，如先前所示（《普通病毒学杂志》[1984]65:639 - 643），在非允许温度下M蛋白的合成减少，与之形成对比的是，L、P、HN、Fo和NP蛋白在允许温度和非允许温度下的合成量相当。在本报告中，我们还表明，由L、P和NP蛋白组成的病毒核衣壳（NC）在这两个温度下的感染细胞内均有形成。在非允许温度下孵育的样品中也检测到了血凝素和神经氨酸酶活性。通过膜免疫荧光和细胞表面免疫沉淀表明，在非允许温度下HN和Fo蛋白向细胞表面的迁移正常发生。此外，与从处于允许温度的感染细胞中分离的质膜相关的L、P和NP蛋白，在非允许温度下孵育的细胞的膜中不存在。这些结果表明，由于缺乏M蛋白，在非允许温度下合成的NC和糖蛋白无法在质膜上有效组装。因此，C6细胞中HVJ的宿主依赖性温度敏感损伤被认为主要在于M蛋白的合成。
