Yoshikawa Y, Yamanouchi K
J Virol. 1984 May;50(2):489-96. doi: 10.1128/JVI.50.2.489-496.1984.
The replication of measles virus in human neural and nonneural cell lines in terms of growth and cytopathic effect was affected by treatment of the cells with papaverine, which increases endogenous cyclic AMP. Suppression of virus growth was most prominent in neuroblastoma cells, followed by that in epidermoid carcinoma and glioblastoma cells, whereas the suppressive effect was relatively weak in oligodendroglioma cells. The papaverine-induced suppression of virus growth in neuroblastoma cells was studied in detail. The suppression that occurred was dependent on the dose of papaverine and was reversible. By treatment with 10 microM papaverine, virus-cell interactions were modified as follows: (i) early replication steps such as adsorption, penetration, and uncoating of the virus were not affected; (ii) synthesis of viral RNAs, including genomic RNA and mRNA, was inhibited; (iii) translation of viral proteins from mRNA was not blocked; and (iv) glycosylation and transport of viral glycoproteins to the cell membrane were not inhibited, but phosphorylation was blocked. The significance of suppressed virus replication in neural cells is discussed in relation to the persistence mechanisms of measles virus in the central nervous system.
用罂粟碱处理细胞会影响麻疹病毒在人神经和非神经细胞系中的复制，包括生长和细胞病变效应，罂粟碱可增加内源性环磷酸腺苷（cAMP）。病毒生长的抑制在神经母细胞瘤细胞中最为显著，其次是表皮样癌细胞和胶质母细胞瘤细胞，而在少突胶质细胞瘤细胞中的抑制作用相对较弱。对罂粟碱诱导的神经母细胞瘤细胞中病毒生长抑制进行了详细研究。所发生的抑制取决于罂粟碱的剂量，并且是可逆的。用10微摩尔罂粟碱处理后，病毒与细胞的相互作用发生如下改变：（i）病毒的早期复制步骤，如吸附、穿透和脱壳不受影响；（ii）包括基因组RNA和mRNA在内的病毒RNA的合成受到抑制；（iii）从mRNA翻译病毒蛋白未被阻断；（iv）病毒糖蛋白的糖基化和向细胞膜的转运未受抑制，但磷酸化被阻断。结合麻疹病毒在中枢神经系统中的持续机制，讨论了神经细胞中病毒复制受抑制的意义。
