Van Alstyne D, Paty D W
Virology. 1983 Jan 15;124(1):173-80. doi: 10.1016/0042-6822(83)90301-x.
Normal adult rat glial (RG) cells in culture have been shown to restrict rubella virus (RV) replication, permitting the synthesis of only five of the seven intracellular viral polypeptides associated with a normal, productive infection. Recently uninfected RG cells have been reported to differentiate into mature glia in the presence of dibutyryl cyclic AMP (dB-cAMP), suggesting that the RG cells may be a continuous cell line of undifferentiated glioblasts. This communication describes the effect of dB-cAMP on rubella-infected RG cells. Following exposure to dB-cAMP, RV antigens could be demonstrated in the cytoplasm of the differentiating glioblasts, all seven intracellular RV polypeptides could be detected following immune precipitation, slab gel electrophoresis, and autoradiography, and infectious progeny virions could be titered from the tissue culture medium. These data indicate that dB-cAMP promotes glioblast maturation and a concomitant activation of RV replication.
培养的正常成年大鼠神经胶质（RG）细胞已被证明可限制风疹病毒（RV）复制，仅允许合成与正常 productive 感染相关的七种细胞内病毒多肽中的五种。最近有报道称，未感染的RG细胞在二丁酰环磷腺苷（dB-cAMP）存在下可分化为成熟神经胶质细胞，这表明RG细胞可能是未分化成胶质母细胞的连续细胞系。本通讯描述了dB-cAMP对风疹感染的RG细胞的影响。暴露于dB-cAMP后，在分化的胶质母细胞的细胞质中可检测到RV抗原，通过免疫沉淀、平板凝胶电泳和放射自显影可检测到所有七种细胞内RV多肽，并且可从组织培养基中滴定出感染性子代病毒粒子。这些数据表明，dB-cAMP促进胶质母细胞成熟并伴随激活RV复制。
