National Research Centre (Scopus Affiliation ID 60014618), Textile Industries Research Division, El-Behouth St., Dokki, P.O. 12622, Giza, Egypt; Biopolymers Interact & Assemblages Research Unit, INRA, F-44316 Nantes, France.
National Research Centre (Scopus Affiliation ID 60014618), Textile Industries Research Division, El-Behouth St., Dokki, P.O. 12622, Giza, Egypt; Department of Forest Biomaterials, College of Natural Resources, North Carolina State University, Raleigh, NC 27695-8005, USA.
Int J Biol Macromol. 2019 Jan;121:814-821. doi: 10.1016/j.ijbiomac.2018.10.081. Epub 2018 Oct 17.
In this study, chitosan/alginate/hydroxyapatite/nanocrystalline cellulose scaffolds were successfully fabricated by the using of freeze-drying method, followed by dicationic crosslinking using CaCl. The chemical structure and morphology along with mechanical properties of the formed scaffolds respecting to various CNC contents were studied by Fourier-transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and mechanical compression test. Chemical interaction and electrostatic attraction between chitosan (CS) and alginate with various CNC ratios were affirmed by FTIR spectroscopy. Results depicted that, scaffolds containing CNC exhibited remarkable improvement in both swelling ratio up to 110% compared without CNC (63%) and compressive strength when compared with other scaffolds. In addition, the average pore size increased, dramatically, with increasing of CNC up to 230 μm. Porosity was also obeyed the sequence and attainted a maximum value at 93.6%. The growth and cell attachment of fibroblast cells of the selected scaffold were examined prolonging to the cell viability by using Alamar Blue (AB) and then confirmed using SEM. The results indicated that the scaffold comprising CNC has a promising cell growth and cell adherence, and thus expected to have a potent possibility for applications in bone tissue culture.
在这项研究中，壳聚糖/海藻酸钠/羟基磷灰石/纳米纤维素晶须支架通过冷冻干燥法成功制备，然后使用 CaCl2进行二价阳离子交联。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和机械压缩试验研究了形成的支架的化学结构和形态以及机械性能，研究了不同 CNC 含量的支架。FTIR 光谱证实了壳聚糖（CS）和海藻酸钠与各种 CNC 比例之间的化学相互作用和静电吸引。结果表明，与不含 CNC（63%）的支架相比，含有 CNC 的支架的溶胀率高达 110%，压缩强度显著提高。此外，随着 CNC 的增加，平均孔径显著增加，最高可达 230µm。孔隙率也遵循该顺序，并在 93.6%时达到最大值。通过使用 Alamar Blue（AB）延长选择的支架的成纤维细胞的生长和细胞附着，并通过 SEM 进一步确认，检查了所选支架的细胞生长和细胞附着。结果表明，包含 CNC 的支架具有良好的细胞生长和细胞附着能力，因此有望在骨组织培养中有潜在的应用可能。
