Polymer Composite Research Laboratory, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Polymer Composite Research Laboratory, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Int J Biol Macromol. 2022 May 31;208:912-924. doi: 10.1016/j.ijbiomac.2022.03.193. Epub 2022 Mar 31.
The effect of glutaraldehyde and calcium cations as covalent and ionic crosslinkers was investigated on the main characteristics of scaffolds based on chitosan and cellulose nanocrystals. Therefore, four different scaffolds based on chitosan/cellulose nanocrystals with different crosslinking methods were fabricated using the freeze-drying method for potential use in bone tissue engineering. The structural and chemical features of prepared scaffolds were studied by the FTIR technique. FESEM images revealed that all scaffold samples are porous three-dimensional networks in which the pores are connected. TGA analysis showed that the thermal stability of scaffolds based on chitosan/cellulose nanocrystals has not been changed significantly by using different crosslinking methods. The chitosan/cellulose nanocrystals scaffold crosslinked by glutaraldehyde represented the highest compressive strength and the uncrosslinked scaffold showed the highest swelling ratio in comparison to the other scaffolds. The fastest degradation rate belonged to the scaffold crosslinked by calcium cations. FESEM images and EDX analysis confirmed that fabricated scaffolds have good biomineralization ability. The cell viability and cell attachment results indicated that all four scaffolds support cell proliferation and cell adhesion. However, the viability of NIH3T3 fibroblast cells in the presence of glutaraldehyde-containing scaffolds was lower than that of other scaffolds.
戊二醛和钙离子作为共价和离子交联剂对壳聚糖和纳米纤维素基支架的主要特性的影响进行了研究。因此，使用冷冻干燥法制备了基于壳聚糖/纳米纤维素的四种不同的支架，这些支架采用不同的交联方法，用于潜在的骨组织工程。通过傅里叶变换红外光谱技术（FTIR）研究了制备支架的结构和化学特征。FESEM 图像显示，所有支架样品均为多孔三维网络，其中孔相互连接。TGA 分析表明，不同交联方法对壳聚糖/纳米纤维素基支架的热稳定性没有显著影响。与其他支架相比，戊二醛交联的壳聚糖/纳米纤维素支架表现出最高的压缩强度，而未交联的支架具有最高的溶胀比。交联钙离子的支架具有最快的降解速率。FESEM 图像和 EDX 分析证实了所制备的支架具有良好的生物矿化能力。细胞活力和细胞附着结果表明，所有四种支架都支持细胞增殖和细胞附着。然而，戊二醛存在时 NIH3T3 成纤维细胞的活力低于其他支架。
