Hinko A, Thibonnier M, Rapp J P
Biochem Biophys Res Commun. 1987 Apr 29;144(2):1076-83. doi: 10.1016/s0006-291x(87)80074-8.
The binding of atrial natriuretic factor (ANF) was studied in kidney membranes of inbred salt-sensitive (S) and inbred salt-resistant (R) rats on high or low salt diet. Important differences between strains were seen in the rate of dissociation of ANF from its renal receptor(s) and this was dependent on salt (NaCl) intake. On low salt diet ANF dissociation rates were similar between strains. R rats responded to high salt diet with a decrease in the rate of ANF dissociation from its renal receptor, but ANF dissociation in S rats was not altered by dietary salt. Receptor density was similar between strains. Basal cGMP production was slightly higher for renal membranes of S rats, but ANF stimulation of cGMP production was similar between strains and was not influenced by salt intake in either strain. Since strain-related salt-induced changes in ANF-receptor binding kinetics were not reflected in any strain-related salt-induced changes in ANF stimulated cGMP production, it is tentatively concluded that the ANF receptor likely to be different between S and R strains is the ANF receptor not linked to cGMP production.
研究了高盐或低盐饮食条件下近交系盐敏感（S）大鼠和近交系盐抵抗（R）大鼠肾膜中的心钠素（ANF）结合情况。在ANF从其肾受体解离的速率方面，品系间存在重要差异，且这取决于盐（氯化钠）摄入量。在低盐饮食时，品系间ANF解离速率相似。R大鼠对高盐饮食的反应是ANF从其肾受体的解离速率降低，但S大鼠的ANF解离不受饮食盐分影响。品系间受体密度相似。S大鼠肾膜的基础环磷酸鸟苷（cGMP）生成略高，但品系间ANF刺激的cGMP生成相似，且两种品系的盐摄入量均未对其产生影响。由于品系相关的盐诱导的ANF受体结合动力学变化未在任何品系相关的盐诱导的ANF刺激的cGMP生成变化中体现，初步得出结论，S和R品系间可能不同的ANF受体是与cGMP生成无关的ANF受体。
