Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
State Key Laboratory of Advanced Special Steel, Shanghai University, Shanghai 200444, PR China.
Biosens Bioelectron. 2019 Jan 15;124-125:115-121. doi: 10.1016/j.bios.2018.09.101. Epub 2018 Oct 9.
For electrochemical biosensors, just like a computer, the modularization and coordinated operation of different components will facilitate the development of versatile biosensors and effectively reduce costs. However, the efficient synergy between different modules is always difficult. It would be a beneficial way to construct the multi-functional module. In this work, a three-dimensional gold nanoparticles/ferrocene/liposome cluster (GFLC) is fabricated and explored as a building block for the fabrication of an electrochemical biosensor, in which gold nanoparticles, ferrocene and liposome cluster work as a signal amplification component, a signal output component and a molecular recognition component, respectively. With the synergy of multi-functions, GFLC has been successfully applied for electrochemical analysis of lipopolysaccharide (LPS) in food samples. LPS can be linearly assayed in the range from 2 × 10 μg/mL to 8 μg/mL with a detection limit of 0.51 × 10 μg/mL. In view of the favorable modularization effect, GFLC shows a great potential in the development of electrochemical biosensor with considerable versatility and cost-efficiency.
对于电化学生物传感器，就像计算机一样，不同组件的模块化和协调运行将有助于开发多功能生物传感器，并有效地降低成本。然而，不同模块之间的高效协同作用总是很困难。构建多功能模块将是一种有益的方法。在这项工作中，构建了一种三维金纳米粒子/二茂铁/脂质体簇（GFLC），并将其探索为电化学生物传感器的构建模块，其中金纳米粒子、二茂铁和脂质体簇分别作为信号放大组件、信号输出组件和分子识别组件。通过多功能的协同作用，GFLC 已成功应用于食品样品中脂多糖（LPS）的电化学分析。LPS 的线性测定范围为 2×10μg/mL 至 8μg/mL，检测限为 0.51×10μg/mL。鉴于良好的模块化效果，GFLC 在开发具有相当多功能性和成本效益的电化学生物传感器方面具有很大的潜力。
