Berkowitz B A, Ackerman J J
Biophys J. 1987 Apr;51(4):681-5. doi: 10.1016/S0006-3495(87)83393-3.
The efficacy of proton decoupling for enhancing the 19F nuclear magnetic resonance (NMR) signal-to-noise ratio and spectral resolution in the intact subject is demonstrated. A geometrically orthogonal cross-coil antenna configuration (Helmholtz pair, surface coil) is employed to provide 40 dB of isolation between the 19F observe and 1H decouple frequencies of 188 and 200 MHz, respectively. Further isolation is achieved through the use of high-quality notch filters on both observation and decoupling channels. Application of 19F-(1H) NMR spectroscopy to the study of 2-fluoro-2-deoxy-D-glucose metabolism in cerebral tissue in situ is presented. Significant improvements in sensitivity and resolution are obtained and result from both a collapse of the JFH multiple structure and a substantial positive nuclear Overhauser effect (NOE). To our knowledge, this is the first such demonstration of 1H decoupling in conjunction with 19F observation for study of the metabolism of a fluorinated compound in the living subject.
质子去耦在增强完整受试者体内19F核磁共振（NMR）信噪比和光谱分辨率方面的功效得到了证实。采用几何正交交叉线圈天线配置（亥姆霍兹对、表面线圈），分别在188 MHz和200 MHz的19F观测频率和1H去耦频率之间提供40 dB的隔离。通过在观测和去耦通道上使用高质量陷波滤波器实现了进一步的隔离。介绍了19F-(1H) NMR光谱在原位研究脑组织中2-氟-2-脱氧-D-葡萄糖代谢中的应用。灵敏度和分辨率得到了显著提高，这是由于JFH多重结构的坍塌和显著的正核Overhauser效应（NOE）。据我们所知，这是首次在活体受试者中结合19F观测进行1H去耦以研究氟化化合物代谢的此类演示。
