Saito M, Tanaka N, Ohkawa Y, Inui N
Cancer Lett. 1987 May;35(2):167-71. doi: 10.1016/0304-3835(87)90041-3.
The inhibitory effects of some scavengers of oxygen radicals were studied, using a two-stage transformation assay system in vitro 3-methylcholanthrene (3-MC)-initiation and 12-O-tetradecanoylphorbol-13-acetate-(TPA)-promotion in Balb 3T3 cells. Mannitol, a scavenger for hydroxyl radicals, strongly inhibited the TPA-enhanced transformation in a dose-dependent manner. Superoxide dismutase (SOD) and catalase, which are specific scavengers for O2-. and H2O2, respectively, also inhibited the transformation. These results suggest that oxygen radicals may play an important role in the TPA-enhanced transformation in Balb 3T3 cells.
利用体外两阶段转化试验系统，即Balb 3T3细胞中的3-甲基胆蒽（3-MC）启动和12-O-十四烷酰佛波醇-13-乙酸酯（TPA）促癌作用，研究了一些氧自由基清除剂的抑制作用。甘露醇是一种羟基自由基清除剂，以剂量依赖方式强烈抑制TPA增强的转化作用。超氧化物歧化酶（SOD）和过氧化氢酶分别是O2-和H2O2的特异性清除剂，它们也抑制转化作用。这些结果表明，氧自由基可能在Balb 3T3细胞中TPA增强的转化作用中发挥重要作用。
