Semba M, Inui N
Laboratory of Cell Toxicology, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Toxicol Lett. 1991 May;56(3):299-303. doi: 10.1016/0378-4274(91)90158-3.
To discover the relationship between the activation of protein kinase C and the generation of reactive oxygen in the tumor promotion process, we investigated the effects of radical scavengers on diacylglycerol-promoted transformation in BALB/3T3 cells. Superoxide dismutase (SOD) showed inhibitory effects on both 1-oleoyl-2-acetylglycerol (OAG)-promoted and diolein-promoted transformation. Catalase (CT) suppressed the promoting effects of diolein by up to 70%. Mannitol (MT), a hydroxyl radical (.OH) scavenger, inhibited diacylglycerol-promoted transformation dose-dependently. These results suggest that activation of protein kinase C alone is insufficient and that generation of reactive oxygen accompanied by activation of the enzyme is essential to the promotion process in BALB/3T3 cells.
为了探究蛋白激酶C的激活与肿瘤促进过程中活性氧生成之间的关系，我们研究了自由基清除剂对BALB/3T3细胞中二酰甘油促进的转化作用的影响。超氧化物歧化酶（SOD）对1-油酰基-2-乙酰甘油（OAG）促进的和二油精促进的转化均显示出抑制作用。过氧化氢酶（CT）可将二油精的促进作用抑制高达70%。羟基自由基（·OH）清除剂甘露醇（MT）剂量依赖性地抑制二酰甘油促进的转化。这些结果表明，仅蛋白激酶C的激活是不够的，并且该酶激活所伴随的活性氧生成对于BALB/3T3细胞的促进过程至关重要。
