Kennedy A R, Troll W, Little J B
Carcinogenesis. 1984 Oct;5(10):1213-8. doi: 10.1093/carcin/5.10.1213.
We have studied the effects of superoxide dismutase (SOD), catalase, Cu(II) (3,5-diisopropylsalicylate)2 (CuDIPS) and other copper compounds on radiation transformation in vitro using C3H 10T1/2 cells. When present only during irradiation, high concentrations of SOD in the medium enhanced transformation, while catalase, inactivated SOD (autoclaved), CuDIPS, cupric chloride and cuprous chloride inhibited the initiation phase of radiation transformation. SOD, catalase and CuDIPS did not affect the expression phase of radiation transformation. Suppression of the TPA enhancement of transformation by catalase was a highly significant effect, while the suppression by SOD was not of statistical significance. Our results suggest that hydrogen peroxide (H2O2) may be important in the cellular damage leading to malignant transformation.
我们使用C3H 10T1/2细胞，研究了超氧化物歧化酶（SOD）、过氧化氢酶、Cu(II) (3,5-二异丙基水杨酸酯)2（CuDIPS）及其他铜化合物对体外辐射转化的影响。当仅在照射期间存在时，培养基中高浓度的SOD会增强转化，而过氧化氢酶、失活的SOD（高压灭菌）、CuDIPS、氯化铜和氯化亚铜会抑制辐射转化的起始阶段。SOD、过氧化氢酶和CuDIPS不影响辐射转化的表达阶段。过氧化氢酶对佛波酯（TPA）增强转化的抑制作用非常显著，而SOD的抑制作用无统计学意义。我们的结果表明，过氧化氢（H2O2）可能在导致恶性转化的细胞损伤中起重要作用。
