Nelson W G, Cho K R, Hsiang Y H, Liu L F, Coffey D S
Cancer Res. 1987 Jun 15;47(12):3246-50.
The relationship between DNA topoisomerase II expression and mammalian cell proliferation has been evaluated by determining enzyme levels in normal and neoplastic rat prostate tissues. By activity assay and by immunoblot analysis using anti-topoisomerase II antiserum, topoisomerase II levels were found to be elevated in both the Dunning R3327-H and the Dunning R3327-G rat prostatic adenocarcinomas over levels assayed in the normal rat dorsal prostate. Immunohistochemical studies using the antitopoisomerase II antiserum revealed that a greater fraction of nuclei contained detectable levels of topoisomerase II in tissue sections prepared from each of the Dunning tumors than in rat dorsal prostate tissue sections. The Dunning R3327-H and R3327-G tumors grow at different rates in vivo (J. T. Isaacs and D. S. Coffey, Clin. Oncol., 2: 479-498, 1983). When measured topoisomerase II levels were compared to known growth parameters for each of the tissues studied, topoisomerase II expression was found to be correlated with tissue growth rate.
通过测定正常和肿瘤大鼠前列腺组织中的酶水平，评估了DNA拓扑异构酶II表达与哺乳动物细胞增殖之间的关系。通过活性测定以及使用抗拓扑异构酶II抗血清的免疫印迹分析，发现邓宁R3327 - H和邓宁R3327 - G大鼠前列腺腺癌中的拓扑异构酶II水平高于正常大鼠背侧前列腺中的测定水平。使用抗拓扑异构酶II抗血清进行的免疫组织化学研究表明，与大鼠背侧前列腺组织切片相比，从每个邓宁肿瘤制备的组织切片中，含有可检测水平拓扑异构酶II的细胞核比例更高。邓宁R3327 - H和R3327 - G肿瘤在体内生长速度不同（J.T.艾萨克斯和D.S.科菲，《临床肿瘤学》，2: 479 - 498，1983）。当将测得的拓扑异构酶II水平与所研究的每个组织的已知生长参数进行比较时，发现拓扑异构酶II表达与组织生长速率相关。
