Li Zhoubin, Zhang Qing, Wu Yutao, Hu Feng, Gu Linling, Chen Ting, Wang Weilin
Department of Lung Transplantation, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Exp Ther Med. 2018 Nov;16(5):3951-3958. doi: 10.3892/etm.2018.6687. Epub 2018 Sep 4.
Airway epithelial cells (AECs) are the first point of contact with airborne antigens and are able to instruct resident immune cells to appropriate immune responses. Previous studies have shown that the abnormal expression of metastasis-associated lung adenocarcinoma transcript 1 (Malat1) was associated with tumorigenesis, progression, metastasis, and apoptosis in many cancer types. However, little is known about its functional involvement in the cross-talk of AECs with dendritic cells (DCs). The aim of the present study was to identify Malat1 as a novel epithelial cell-derived immune-modulating factor that contributes to the specific inflammatory-immune airway microenvironment. By using an co-culture model, where layers of AECs can interact with DCs, and transfecting Malat1 siRNA in AECs, AEC-conditioned DCs were harvested for further analysis of the celluar phenotype, secretion of inflammatory chemokines, and expression of apoptotic markers. The present study clearly demonstrated that Malat1 modulates the maturation process, pro-inflammatory cytokine secretion and apoptosis in AECs-conditioned DCs.
气道上皮细胞（AECs）是与空气传播抗原的首个接触点，并且能够指导驻留免疫细胞产生适当的免疫反应。先前的研究表明，转移相关肺腺癌转录本1（Malat1）的异常表达与多种癌症类型的肿瘤发生、进展、转移和凋亡相关。然而，关于其在AECs与树突状细胞（DCs）相互作用中的功能作用知之甚少。本研究的目的是确定Malat1作为一种新型上皮细胞衍生的免疫调节因子，它有助于形成特定的炎症免疫气道微环境。通过使用一种共培养模型，其中AECs层可以与DCs相互作用，并在AECs中转染Malat1小干扰RNA，收获经AECs处理的DCs，以进一步分析细胞表型、炎症趋化因子的分泌以及凋亡标志物的表达。本研究清楚地表明，Malat1调节经AECs处理的DCs的成熟过程、促炎细胞因子分泌和凋亡。
