Fuentes-Valdés Juan J, Soto-Liebe Katia, Pérez-Pantoja Danilo, Tamames Javier, Belmar Lucy, Pedrós-Alió Carlos, Garrido Daniel, Vásquez Mónica
1Department of Molecular Genetics and Microbiology, Pontificia Universidad Católica de Chile, 8331150 Santiago, Chile.
2Department of Chemical and Bioprocess Engineering, Pontificia Universidad Católica de Chile, 7820436 Santiago, Chile.
Stand Genomic Sci. 2018 Oct 12;13:26. doi: 10.1186/s40793-018-0323-1. eCollection 2018.
Members of the genus represent an important environmental and health concern. Strains CS-508 and MVCC14 of were isolated from freshwater reservoirs located in Australia and Uruguay, respectively. While CS-508 has been reported as non-toxic, MVCC14 is a saxitoxin (STX) producer. We annotated the draft genomes of these strains using the assembly of reads obtained from Illumina MiSeq sequencing. The final assemblies resulted in genome sizes close to 3.6 Mbp for both strains and included 3202 ORFs for CS-508 (in 163 contigs) and 3560 ORFs for MVCC14 (in 99 contigs). Finally, both the average nucleotide identity (ANI) and the similarity of gene content indicate that these two genomes should be considered as strains of the species.
该属的成员代表了一个重要的环境和健康问题。分别从位于澳大利亚和乌拉圭的淡水水库中分离出了该属的CS - 508菌株和MVCC14菌株。虽然CS - 508已被报道为无毒，但MVCC14是一种石房蛤毒素（STX）产生菌。我们使用从Illumina MiSeq测序获得的 reads 组装对这些菌株的基因组草图进行了注释。最终组装结果显示，这两个菌株的基因组大小均接近3.6 Mbp，其中CS - 508包含3202个开放阅读框（位于163个重叠群中），MVCC14包含3560个开放阅读框（位于99个重叠群中）。最后，平均核苷酸同一性（ANI）和基因含量相似性均表明，这两个基因组应被视为该物种的菌株。
