Barbers R G, Oishi J
Environ Res. 1987 Jun;43(1):217-26. doi: 10.1016/s0013-9351(87)80073-7.
Human peripheral blood lymphocytes (PBL) were exposed in vitro to asbestos fibers. Antibody-dependent cellular cytotoxicity (ADCC) activity and natural killer (NK) activity were examined by a chromium-51 release assay. There was a statistically significant enhancement of ADCC and NK activity by chrysotile and crocidolite fibers when cultured together with PBL for a period of 42 hr in medium containing a concentration of at least 2.5% fetal calf serum (P less than 0.05). Isolation of large granular lymphocytes to measure NK activity, however, showed the opposite effect when exposed to asbestos fibers (P less than 0.05). Our results indicate that asbestos fibers can directly affect lymphoid cytotoxic responses in vitro and may provide clues to immunopathogenic mechanisms for the occurrence of neoplasms in vivo.
将人外周血淋巴细胞（PBL）在体外暴露于石棉纤维。通过铬-51释放试验检测抗体依赖性细胞毒性（ADCC）活性和自然杀伤（NK）活性。当温石棉和青石棉纤维与PBL在含有至少2.5%胎牛血清的培养基中共同培养42小时时，ADCC和NK活性有统计学意义的增强（P<0.05）。然而，分离大颗粒淋巴细胞以测量NK活性时，暴露于石棉纤维时显示出相反的效果（P<0.05）。我们的结果表明，石棉纤维可在体外直接影响淋巴细胞毒性反应，并可能为体内肿瘤发生的免疫致病机制提供线索。
