Nakatani Y
Sangyo Igaku. 1983 Sep;25(5):375-86. doi: 10.1539/joh1959.25.375.
The biological effects of asbestos and glass fibers on lymphocytes in vitro were investigated. Blastoid transformation and beta 2 microglobulin production of human peripheral blood lymphocytes (PBL) induced by phytohemagglutinin were inhibited by Canadian chrysotile B (Standard sample of the Union Internationale Contre le Cancer; UICC standard sample) but it was not the case for crocidolite (UICC standard sample) and small and large glass fibers (John Manville, Canada). Cytotoxic activities of natural killer and killer cells of PBL were investigated using K562 cells and Chang liver cells as target cells respectively. Chrysotile inhibited the both activities but crocidolite and two kind of glass fibers not. In regard to release of lactic dehydrogenase and beta-glucuronidase from mouse peritoneal macrophages exposed to mineral fibers, chrysotile showed more effective reaction compared to crocidolite and amosite (UICC standard sample), on the other hand large glass fiber showed the similar reaction to chrysotile but small glass fiber and milled amosite did not induce any more enzymatic release than the control. In addition to chemical compositions of mineral fibers, the morphological characteristics were also discussed in relation to their biological effects.
研究了石棉和玻璃纤维在体外对淋巴细胞的生物学效应。加拿大温石棉B（国际抗癌联盟标准样品；UICC标准样品）可抑制植物血凝素诱导的人外周血淋巴细胞（PBL）的母细胞样转化和β2微球蛋白产生，但青石棉（UICC标准样品）以及大小玻璃纤维（加拿大约翰·曼维尔公司）则无此作用。分别以K562细胞和Chang肝细胞为靶细胞，研究了PBL的自然杀伤细胞和杀伤细胞的细胞毒活性。温石棉可抑制这两种活性，但青石棉和两种玻璃纤维则无此作用。关于暴露于矿物纤维的小鼠腹腔巨噬细胞释放乳酸脱氢酶和β-葡萄糖醛酸酶，与青石棉和铁石棉（UICC标准样品）相比，温石棉表现出更有效的反应，另一方面，大玻璃纤维表现出与温石棉相似的反应，但小玻璃纤维和磨碎的铁石棉诱导的酶释放量并不比对照更多。除了矿物纤维的化学成分外，还讨论了其形态特征与其生物学效应的关系。
