Centre for Ophthalmology and Visual Science and the Lions Eye Institute, University of Western Australia, Nedlands, Western Australia, Australia.
Department of Ophthalmology, University of New South Wales, Kensington, New South Wales, Australia.
Clin Exp Ophthalmol. 2019 May;47(4):445-452. doi: 10.1111/ceo.13423. Epub 2018 Nov 14.
Excessive ocular sun exposure is linked to various eye pathologies. Conjunctival ultraviolet autofluorescence (CUVAF) is a method of detecting sun-related conjunctival damage; however, the custom-built camera system required is not readily available.
We investigated whether blue laser autofluorescence (BAF) on a commonly used confocal scanning laser ophthalmoscope (cSLO) can be utilized to measure CUVAF area.
Cross-sectional evaluation of a diagnostic technology at a medical research institute.
Sixty-four participants recruited from three on-going observational eye studies in Western Australia.
All participants had four images, two of each eye, captured using the CUVAF camera and BAF on the same day. Participants with pterygium or poor quality images were excluded from the analysis. Two graders measured CUVAF area in each image twice. CUVAF area measured by BAF was then compared to measurements determined with the conventional camera system.
CUVAF area.
After exclusions, 50 participants' images were analysed. Intra- and inter-observer repeatability were similar between the two systems. When comparing CUVAF area measured by BAF to the camera measurement, grader 1 had a mean difference of +1.00 mm , with 95% limits of agreement -5.75 to 7.77 mm . Grader 2 had a mean difference of +0.21mm , with 95% limits of agreement -7.22 to 7.64 mm .
BAF on a commercially available cSLO is a valid method for measuring CUVAF area. This finding provides broader opportunity for identifying, monitoring and educating patients with sun-exposure-related ocular conditions and for researching the ocular impacts of sun exposure.
过度的眼部阳光暴露与各种眼部疾病有关。结膜紫外线自发荧光（CUVAF）是一种检测与阳光有关的结膜损伤的方法；然而，所需的定制相机系统并不容易获得。
我们研究了常用共焦扫描激光检眼镜（cSLO）上的蓝色激光自发荧光（BAF）是否可用于测量 CUVAF 面积。
在医学研究所对诊断技术进行横断面评估。
从西澳大利亚州的三项正在进行的眼部观察研究中招募的 64 名参与者。
所有参与者在同一天使用 CUVAF 相机和 BAF 拍摄了四张图像，每张眼睛各两张。有翼状胬肉或图像质量差的参与者被排除在分析之外。两位评分员两次测量了每张图像中的 CUVAF 面积。然后将 BAF 测量的 CUVAF 面积与常规相机系统的测量结果进行比较。
CUVAF 面积。
排除后，分析了 50 名参与者的图像。两种系统的观察者内和观察者间重复性相似。当将 BAF 测量的 CUVAF 面积与相机测量结果进行比较时，评分员 1 的平均差异为+1.00mm，95%一致性界限为-5.75 至 7.77mm。评分员 2 的平均差异为+0.21mm，95%一致性界限为-7.22 至 7.64mm。
商业 cSLO 上的 BAF 是测量 CUVAF 面积的有效方法。这一发现为识别、监测和教育与阳光暴露相关的眼部疾病患者以及研究阳光暴露对眼部的影响提供了更广泛的机会。
