Miyamoto T, Lindgren J A, Hökfelt T, Samuelsson B
FEBS Lett. 1987 May 25;216(1):123-7. doi: 10.1016/0014-5793(87)80769-x.
The regional distribution of ionophore A23187-induced synthesis of leukotrienes and mono-hydroxyeicosatetraenoic acids in the rat brain in vitro was investigated. Pronounced differences in leukotriene C4 formation were observed, with the highest synthetic capacity in the hypothalamus. The formation of leukotriene C4 was about 12-times higher in the hypothalamus as compared to the cerebellum. This finding is in agreement with a possible neuroendocrine role for leukotriene C4. In contrast, the activity of leukotriene B4 synthesis was widely distributed without pronounced regional differences in the rat brain. Formation of 5-, 9-, 11-, 12- and 15-monohydroxyeicosatetraeonoic acid was detected in all regions. The major lipoxygenase product in the hypothalamus and thalamus was 5-hydroxyeicosatetraenoic acid, while other monohydroxyeicosatetraenoic acids predominated in the remaining regions tested.
研究了离子载体A23187在体外诱导大鼠脑内白三烯和单羟基二十碳四烯酸合成的区域分布。观察到白三烯C4形成存在明显差异，下丘脑的合成能力最高。下丘脑白三烯C4的形成比小脑高约12倍。这一发现与白三烯C4可能的神经内分泌作用一致。相比之下，白三烯B4合成活性分布广泛，在大鼠脑中无明显区域差异。在所有区域均检测到5-、9-、11-、12-和15-单羟基二十碳四烯酸的形成。下丘脑和丘脑的主要脂氧合酶产物是5-羟基二十碳四烯酸，而在其余测试区域中其他单羟基二十碳四烯酸占主导。
