[Insertion mutagenesis in Bacillus subtilis (Marmur)].
作者信息
Stoilova-Disheva M M, Prozorov A A
出版信息
Genetika. 1987 Apr;23(4):616-21.
PMID:3034723
Abstract
A method for obtaining mutations in Bacillus subtilis using integration into the chromosome of the plasmid pHV60 ligated to chromosomal DNA fragments was developed. Auxotrophic mutants acquire, in addition, chloramphenicol-resistance, due to insertion of appropriate plasmid determinants. Chromosomal localization was established and the properties of several mutants were studied.
摘要
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