Kariya Y, Kato K, Hayashizaki Y, Himeno S, Tarui S, Matsubara K
Gene. 1986;50(1-3):345-52. doi: 10.1016/0378-1119(86)90338-0.
The complete sequence of the human gastrin gene is reported here. This gene consists of three exons. Nine Alu family sequences are found within the gene and in the surrounding region. S1 mapping study showed that the transcription of gastrin gene starts at 60, 57, or 55 bp upstream from the 3' end of the first exon. The mechanism of mRNA synthesis in a gastrinoma tissue was studied to clarify the ectopic production of gastrin. It was found that mRNA synthesis starts from the same three transcriptional start points. Southern blotting profiles for normal gastric antrum and gastrinoma DNA were indistinguishable from each other within the 18-kb region containing the gastrin gene, showing that no genomic rearrangements are associated with the gastrinoma formation. Thus, the overproduction of gastrin in this tumor is likely to be due to an aberrant expression control system of the cell, rather than a change in the control region of the gastrin gene.
本文报道了人胃泌素基因的完整序列。该基因由三个外显子组成。在该基因及其周边区域发现了九个Alu家族序列。S1图谱研究表明，胃泌素基因的转录起始于第一个外显子3'端上游60、57或55 bp处。为阐明胃泌素的异位产生，对胃泌素瘤组织中mRNA合成机制进行了研究。发现mRNA合成起始于相同的三个转录起始位点。在包含胃泌素基因的18 kb区域内，正常胃窦和胃泌素瘤DNA的Southern印迹图谱彼此无法区分，表明胃泌素瘤形成与基因组重排无关。因此，该肿瘤中胃泌素的过量产生可能是由于细胞异常的表达控制系统，而非胃泌素基因调控区域的改变。
