Chittepu Veera C S R, Kalhotra Poonam, Gallardo-Velázquez Tzayhri, Robles-de la Torre Raúl René, Osorio-Revilla Guillermo
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu S/N, Col. Unidad Profesional Adolfo López Mateos, Zacatenco, CP. Ciudad de Mexico 07738, Mexico.
Departamento de Biofísica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Prolongación de Carpio y Plan de Ayala S/N, Col. Santo Tomás, CP. Ciudad de Mexico 11340, Mexico.
Nanomaterials (Basel). 2018 Oct 19;8(10):852. doi: 10.3390/nano8100852.
Functionalized single-walled carbon nanotubes with polyethylene glycol (PEGylated SWCNTs) are a promising nanomaterial that recently has emerged as the most attractive "cargo" to deliver chemicals, peptides, DNA and RNAs into cells. Insulin therapy is a recommended therapy to treat diabetes mellitus despite its side effects. Recently, functional dispersion made up of bioactive peptides, bioactive compounds and functionalized carbon nanomaterials such as PEGylated SWCNTs have proved to possess promising applications in nanomedicine. In the present study, molecular modeling simulations are utilized to assist in designing insulin hormone-PEGylated SWCNT composites, also called functional dispersion; to achieve this experimentally, an ultrasonication tool was utilized. Enzymatic degradation assay revealed that the designed functional dispersion protects about 70% of free insulin from pepsin. In addition, sulforhodamine B (SRB) assay, the quantification of insulin and glucose levels in differentiated skeletal muscle cell supernatants, reveals that functional dispersion regulates glucose and insulin levels to promote skeletal muscle cell proliferation. These findings offer new perspectives for designed functional dispersion, as potential pharmaceutical preparations to improve insulin therapy and promote skeletal muscle cell health.
聚乙二醇功能化单壁碳纳米管（PEG化单壁碳纳米管）是一种很有前景的纳米材料，最近已成为将化学物质、肽、DNA和RNA输送到细胞中最具吸引力的“载体”。胰岛素疗法是治疗糖尿病的推荐疗法，尽管有副作用。最近，由生物活性肽、生物活性化合物和功能化碳纳米材料（如PEG化单壁碳纳米管）组成的功能分散体已被证明在纳米医学中具有广阔的应用前景。在本研究中，利用分子模拟来辅助设计胰岛素-PEG化单壁碳纳米管复合材料，也称为功能分散体；为了通过实验实现这一点，使用了超声工具。酶降解试验表明，所设计的功能分散体可保护约70%的游离胰岛素不被胃蛋白酶降解。此外，磺酰罗丹明B（SRB）试验、分化的骨骼肌细胞上清液中胰岛素和葡萄糖水平的定量分析表明，功能分散功能分散体可调节葡萄糖和胰岛素水平，以促进骨骼肌细胞增殖。这些发现为设计的功能分散体提供了新的视角，作为潜在的药物制剂可改善胰岛素治疗并促进骨骼肌细胞健康。
