Newell Ryan, Wang Zi, Arias Isabel, Mehta Abhishek, Sohn Yongho, Florczyk Stephen
Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816-2455, USA.
Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32827, USA.
J Funct Biomater. 2018 Oct 19;9(4):59. doi: 10.3390/jfb9040059.
Transition metal multi-principal element alloys (MPEAs) are novel alloys that may offer enhanced surface and mechanical properties compared with commercial metallic alloys. However, their biocompatibility has not been investigated. In this study, three CoCrFeNi-based MPEAs were fabricated, and the in vitro cytotoxicity was evaluated in direct contact with fibroblasts for 168 h. The cell viability and cell number were assessed at 24, 96, and 168 h using LIVE/DEAD assay and alamarBlue assay, respectively. All MPEA sample wells had a high percentage of viable cells at each time point. The two quaternary MPEAs demonstrated a similar cell response to stainless steel control with the alamarBlue assay, while the quinary MPEA with Mn had a lower cell number after 168 h. Fibroblasts cultured with the MPEA samples demonstrated a consistent elongated morphology, while those cultured with the Ni control samples demonstrated changes in cell morphology after 24 h. No significant surface corrosion was observed on the MPEAs or stainless steel samples following the cell culture, while the Ni control samples had extensive corrosion. The cell growth and viability results demonstrate the cytocompatibility of the MPEAs. The biocompatibility of MPEAs should be investigated further to determine if MPEAs may be utilized in orthopedic implants and other biomedical applications.
过渡金属多主元合金（MPEAs）是一种新型合金，与商用金属合金相比，其表面性能和机械性能可能更优。然而，其生物相容性尚未得到研究。在本研究中，制备了三种钴铬铁镍基MPEAs，并评估了它们与成纤维细胞直接接触168小时后的体外细胞毒性。分别在24、96和168小时使用活/死细胞检测法和alamarBlue检测法评估细胞活力和细胞数量。在每个时间点，所有MPEA样品孔中活细胞的比例都很高。alamarBlue检测法显示，两种四元MPEAs对不锈钢对照的细胞反应相似，而含锰的五元MPEA在168小时后细胞数量较少。与MPEA样品共培养的成纤维细胞呈现出一致的伸长形态，而与镍对照样品共培养的成纤维细胞在24小时后细胞形态发生了变化。细胞培养后，未观察到MPEAs或不锈钢样品有明显的表面腐蚀，而镍对照样品有广泛的腐蚀。细胞生长和活力结果证明了MPEAs的细胞相容性。应进一步研究MPEAs的生物相容性，以确定MPEAs是否可用于骨科植入物和其他生物医学应用。
