College of Chemical Engineering, Fuzhou University, Fuzhou 350108, China.
College of Chemical Engineering, Fuzhou University, Fuzhou 350108, China.
Talanta. 2019 Jan 15;192:14-23. doi: 10.1016/j.talanta.2018.08.084. Epub 2018 Aug 31.
Bovine serum albumin (BSA) imprinted polyampholyte hydrogels (PAHs) were prepared by free radical polymerization using acrylamide (Am) as structural monomer, N-isopropylacrylamide (NIPAm), [2-(methacryloyloxy)ethyl]trimethylammonium chloride (DMC) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) as functional monomers and N,N'-methylenebisacrylamide (MBA) as crosslinker in aqueous solution. The morphology of imprinted hydrogels and non-imprinted hydrogels were characterized by scanning electron microscope (SEM). The adsorption and recognition properties were evaluated as functions of Am monomer concentration, NIPAm/Am molar ratio, crosslinking structure and charge density ratio etc. The adsorption capacity and association constant of specific interaction between hydrogel and template protein were analyzed by Langmuir isotherm model and Freundlich model. The fitting experimental data suggested that this adsorption was better described as a monolayer adsorption. The specific adsorption on hydrogel with different crosslinking structure was investigated by selective binding BSA from single solution and binary mixture solution. The charge density ratio in molecular imprinting hydrogel had obvious influence for protein adsorption and recognition. The resultant of regeneration tests showed that elution had large impact on deterioration of the imprinting structure.
牛血清白蛋白（BSA）印迹聚两性电解质水凝胶（PAHs）通过自由基聚合，以丙烯酰胺（Am）为结构单体、N-异丙基丙烯酰胺（NIPAm）、[2-（甲基丙烯酰氧基）乙基]三甲基氯化铵（DMC）和 2-丙烯酰胺基-2-甲基-1-丙磺酸（AMPS）为功能单体和 N,N'-亚甲基双丙烯酰胺（MBA）为交联剂在水溶液中制备。印迹水凝胶和非印迹水凝胶的形态用扫描电子显微镜（SEM）进行了表征。通过Langmuir 等温线模型和 Freundlich 模型分析了印迹水凝胶与模板蛋白之间的特异性相互作用的吸附容量和结合常数。拟合实验数据表明，这种吸附更适合单层吸附。通过从单一溶液和二元混合物溶液中选择性结合 BSA 来研究具有不同交联结构的水凝胶的特异性吸附。分子印迹水凝胶中的电荷密度比对蛋白质的吸附和识别有明显的影响。再生试验的结果表明，洗脱对印迹结构的恶化有很大的影响。
