College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China.
Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Shanghai), Institute for Agro-Food Standards and Testing Technology, Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Molecules. 2021 Apr 13;26(8):2243. doi: 10.3390/molecules26082243.
Deoxynivalenol (DON) is one of the most common mycotoxins in grains, causing gastrointestinal inflammation, neurotoxicity, hepatotoxicity and embryotoxicity, even at a low quantity. In this study, a facile electrochemical aptasensor was established for the rapid and sensitive determination of DON based on a multifunctional N-doped Cu-metallic organic framework (N-Cu-MOF) nanomaterial. The N-Cu-MOF, with a large specific surface area and good electrical conductivity, served not only as an optimal electrical signal probe but also as an effective supporting substrate for stabilizing aptamers through the interactions of amino (-NH) and copper. Under the optimal conditions, the proposed sensor provided a wide linear concentration range of 0.02-20 ng mL (R = 0.994), showing high sensitivity, with a lower detection limit of 0.008 ng mL, and good selectivity. The sensor's effectiveness was also verified in real spiked wheat samples with satisfactory recoveries of 95.6-105.9%. The current work provides a flexible approach for the rapid and sensitive analysis of highly toxic DON in food samples and may also be easily extended to detect other hazardous substances with alternative target-recognition aptamers.
脱氧雪腐镰刀菌烯醇（DON）是谷物中最常见的霉菌毒素之一，即使在低浓度下，也会引起胃肠道炎症、神经毒性、肝毒性和胚胎毒性。在这项研究中，基于多功能氮掺杂铜金属有机骨架（N-Cu-MOF）纳米材料，建立了一种用于快速灵敏检测 DON 的简便电化学适体传感器。N-Cu-MOF 具有较大的比表面积和良好的导电性，不仅可用作最佳电信号探针，而且还可以通过氨基（-NH）和铜的相互作用作为稳定适体的有效支撑基底。在最佳条件下，所提出的传感器提供了 0.02-20 ng mL 的宽线性浓度范围（R = 0.994），具有高灵敏度，检测限低至 0.008 ng mL，且具有良好的选择性。该传感器在实际添加小麦样品中的有效性也得到了验证，回收率为 95.6-105.9%。目前的工作为快速灵敏分析食品样品中的高毒性 DON 提供了一种灵活的方法，并且可以很容易地扩展到使用替代靶标识别适体来检测其他有害物质。
