Department of Analytical Chemistry, Institute of Biomolecules (INBIO), Faculty of Sciences, CEI-MAR, University of Cadiz, Campus Rio San Pedro, ES-11510 Puerto Real, Cadiz, Spain.
Department of Analytical Chemistry, Institute of Biomolecules (INBIO), Faculty of Sciences, CEI-MAR, University of Cadiz, Campus Rio San Pedro, ES-11510 Puerto Real, Cadiz, Spain.
Talanta. 2019 Jan 15;192:374-379. doi: 10.1016/j.talanta.2018.09.040. Epub 2018 Sep 15.
A tandem system based on the coupling of a bulk liquid membrane and a flow injection analysis for the separation, preconcentration and spectrophotometric determination of copper in saline water is presented. The ligand pyridine-2-acetaldehyde benzoylhydrazone has been used as a carrier in the liquid membrane as well as a spectrophotometric reagent for UV-VIS detection. Simultaneous and sequential experimental designs were used to optimise the chosen variables of each technique, respectively. The metal was separated and preconcentrated from the sample with an efficiency of 100.5 ± 0.9% and a metal preconcentration factor of 16.1. The on-line FIA determination was accomplished after metal complexation by the reagent at pH 3. A linear response was obtained in a range from 6.9 to 984.5 µg L Cu(II), providing a detection limit of 1.8 µg L. Saline matrix and other metal ions were not cause of interferences with relative errors below 4.6% for 50 µg L of Cu(II) determination. The proposed tandem system was successfully tested using a TMDA-62 certified reference material providing a relative error of + 1.9%; it was also applied to the Cu(II) determination in coastal seawater samples with low relative errors ranging from - 3.8% to 0.0% (using DPASV as reference method).
提出了一种基于 bulk liquid membrane 和 flow injection analysis 偶联的串联系统，用于分离、预浓缩和分光光度法测定盐水中的铜。配体吡啶-2-乙醛苯甲酰腙被用作液体膜中的载体以及用于 UV-VIS 检测的分光光度试剂。分别使用同时和顺序实验设计来优化每种技术的选定变量。金属在 100.5 ± 0.9%的效率和 16.1 的金属预浓缩因子下从样品中分离和预浓缩。金属络合物在 pH 3 下由试剂在线完成 FIA 测定。在线 FIA 测定在 6.9 至 984.5 µg/L Cu(II)范围内获得线性响应，检测限为 1.8 µg/L。盐基质和其他金属离子不会引起干扰，对于 50 µg/L 的 Cu(II)测定，相对误差低于 4.6%。该串联系统使用 TMDA-62 认证参考物质成功进行了测试，相对误差为+1.9%；它还被应用于沿海海水样品中 Cu(II)的测定，相对误差范围为-3.8%至 0.0%（使用 DPASV 作为参考方法）。
