Darlington G J, Kelly J H, Buffone G J
In Vitro Cell Dev Biol. 1987 May;23(5):349-54. doi: 10.1007/BF02620991.
The production of albumin, alpha-fetoprotein (AFP), and alpha-1 antitrypsin has been compared among human hepatoma cells cultured in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo. in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media. Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount of secreted albumin whereas insulin at 10 micrograms/ml reduced the level of total secreted protein.
已对在含血清培养基、含激素和生长因子培养基以及仅添加硒作为唯一补充剂的基础培养基中培养的人肝癌细胞中白蛋白、甲胎蛋白（AFP）和α1抗胰蛋白酶的产生情况进行了比较。在所有三种培养基中细胞生长均得以维持，并且在不同培养基中这三种蛋白质的表达均维持了4个多月。然而，三种培养基中白蛋白和AFP的定量产生存在显著差异。两种激素，即胰岛素和三碘甲状腺原氨酸，影响分泌蛋白的水平。三碘甲状腺原氨酸增加分泌白蛋白的量，而10微克/毫升的胰岛素降低总分泌蛋白的水平。
