Aden D P, Fogel A, Plotkin S, Damjanov I, Knowles B B
Nature. 1979 Dec 6;282(5739):615-6. doi: 10.1038/282615a0.
A significant aspect of primary hepatic carcinoma in man is the high positive correlation of hepatocellular carcinoma with infection with hepatitis B virus (HBV)1. Analysis of the relationship between HBV infection and oncogenesis is difficult because natural infection with HBV is limited to man and experimental infection has been achieved only in chimpanzees and gibbons. Furthermore, because HBV has not been successfully propagated in cell culture, basic study of virus-cell interaction of the aetiological agent of one of the most widespread infections of man has been impossible. Recently, however, a cell line (PLC/PRF/5) derived from a human hepatoma biopsy was described which produces the HRV surface antigen (HBsAg) and so provides a tool for the experimental investigation of HBV in viro. We now report the derivation and characterisation of two additional cell lines primary liver carcinomas. In contrast to the PLC/PRF/5 cell line, these cell lines retain the capacity to synthesise many human plasma proteins, including both albumin and alpha-fetoprotein (AFP). One of these lines also produces BHsAg. We also present evidence that HBsAg synthesis and secretion in this cell line are correlated with the growth state of the culture. This finding is in contrast to the continuous HBsAg production found in the PLC/PRF/5 cell line.
人类原发性肝癌的一个重要方面是肝细胞癌与乙型肝炎病毒（HBV）感染之间存在高度正相关。分析HBV感染与肿瘤发生之间的关系很困难，因为HBV的自然感染仅限于人类，而实验性感染仅在黑猩猩和长臂猿中实现。此外，由于HBV尚未在细胞培养中成功繁殖，因此对人类最广泛感染之一的病原体的病毒-细胞相互作用进行基础研究是不可能的。然而，最近描述了一种源自人肝癌活检的细胞系（PLC/PRF/5），它能产生乙肝病毒表面抗原（HBsAg），因此为体外实验研究HBV提供了一种工具。我们现在报告另外两种原发性肝癌细胞系的衍生和特性。与PLC/PRF/5细胞系不同，这些细胞系保留了合成许多人类血浆蛋白的能力，包括白蛋白和甲胎蛋白（AFP）。其中一个细胞系也产生HBsAg。我们还提供证据表明，该细胞系中HBsAg的合成和分泌与培养物的生长状态相关。这一发现与PLC/PRF/5细胞系中持续产生HBsAg的情况形成对比。
