低强度脉冲超声可诱导软骨细胞合成软骨基质并减少 MMP13 的表达。

Low-intensity pulsed ultrasound induces cartilage matrix synthesis and reduced MMP13 expression in chondrocytes.

机构信息

Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan.

Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Canada.

出版信息

Biochem Biophys Res Commun. 2018 Nov 17;506(1):290-297. doi: 10.1016/j.bbrc.2018.10.063. Epub 2018 Oct 19.

DOI:10.1016/j.bbrc.2018.10.063

PMID:30348521

Abstract

Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics. In previous in vivo and in vitro studies, LIPUS has been shown to have promising effects on cellular elements in articular cartilage, particularly chondrocytes in patients with osteoarthritis. However, the effects of LIPUS on the cellular mechanisms through which LIPUS alters extracellular matrix (ECM) synthesis in chondrocytes are unclear. In this study, we investigated the effects of the optimal intensity and cellular mechanisms of LIPUS on the regeneration of cartilage matrix in chondrocytes. LIPUS induced collagen synthesis and the remodeling of aggrecan via the activation of ERK1/2. In contrast, MMP13 expression was decreased in chondrocytes. Additionally, chondrocytes responded optimally to LIPUS at an intensity higher than the clinical setting for bone fracture healing. These results suggested that LIPUS induced ECM regeneration via increases in hypertrophic chondrocytes and delayed endochondral ossification in chondrocytes.

摘要

低强度脉冲超声（LIPUS）用于骨科的骨骼愈合。在以前的体内和体外研究中，LIPUS 已被证明对关节软骨中的细胞成分具有有前景的作用，特别是对骨关节炎患者的软骨细胞。然而，LIPUS 对细胞机制的影响，即 LIPUS 改变软骨细胞细胞外基质（ECM）合成的机制尚不清楚。在这项研究中，我们研究了 LIPUS 的最佳强度和细胞机制对软骨细胞中软骨基质再生的影响。LIPUS 通过激活 ERK1/2 诱导胶原蛋白合成和聚集蛋白聚糖的重塑。相反，MMP13 的表达在软骨细胞中降低。此外，软骨细胞对高于骨折愈合的临床设定强度的 LIPUS 反应最佳。这些结果表明，LIPUS 通过增加肥大软骨细胞和延迟软骨细胞的软骨内骨化来诱导 ECM 再生。