香叶基香叶醇诱导PPARγ表达并增强PPARγ激动剂在脂肪细胞系细胞中的生物学效应。
Geranylgeraniol Induces PPARγ Expression and Enhances the Biological Effects of a PPARγ Agonist in Adipocyte Lineage Cells.
作者信息
Matsubara Takuma, Takakura Nana, Urata Mariko, Muramatsu Yuya, Tsuboi Makoto, Yasuda Kazuma, Addison William N, Zhang Min, Matsuo Kou, Nakatomi Chihiro, Shigeyama-Tada Yukiyo, Kaneuji Takeshi, Nakamichi Atsuko, Kokabu Shoichiro
机构信息
Division of Molecular Signaling and Biochemistry, School of Oral Health Sciences, Kyushu Dental University, Kitakyushu, Japan.
Research Unit, Shriners Hospitals for Children-Canada, Department of Human Genetics, McGill University, Montreal, QC, Canada.
出版信息
In Vivo. 2018 Nov-Dec;32(6):1339-1344. doi: 10.21873/invivo.11384.
BACKGROUND
The global incidence of diabetes mellitus (DM) has risen precipitously, even in middle- and low-income countries. Peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in the control of cellular glucose metabolism. Activation of PPARγ beneficially results in increased insulin sensitivity. However, the expression of PPARγ is reduced by obesity and several nutritional factors. Here we examined the effect of geranylgeraniol (GGOH), a bioactive compound found naturally in fruits, vegetables, and grains, on the expression and activation of PPARγ.
MATERIALS AND METHODS
C3H10T1/2 mouse embryonic fibroblasts and 3T3-L1 pre-adipocytes were used as in vitro models of adipocyte differentiation and function. Quantitative reverse-transcriptase polymerase chain reaction, western blotting, Oil Red O staining, and luciferase assay were performed to respectively assess mRNA expression, protein levels, lipid droplet formation and transcriptional activity.
RESULTS
GGOH increased the expression of PPARγ in adipocyte lineage cells. GGOH also enhanced adipogenesis induced by rosiglitazone, a thiazolidinedione class PPARγ agonist.
CONCLUSION
GGOH induces PPARγ expression and enhances the biological effects of a PPARγ agonist in adipocyte lineage cells.
背景
糖尿病（DM）的全球发病率急剧上升，即使在中低收入国家也是如此。过氧化物酶体增殖物激活受体γ（PPARγ）在细胞葡萄糖代谢的控制中起重要作用。PPARγ的激活有益地导致胰岛素敏感性增加。然而，肥胖和几种营养因素会降低PPARγ的表达。在此，我们研究了香叶基香叶醇（GGOH），一种天然存在于水果、蔬菜和谷物中的生物活性化合物，对PPARγ表达和激活的影响。
材料与方法
将C3H10T1/2小鼠胚胎成纤维细胞和3T3-L1前脂肪细胞用作脂肪细胞分化和功能的体外模型。进行定量逆转录聚合酶链反应、蛋白质印迹、油红O染色和荧光素酶测定，以分别评估mRNA表达、蛋白质水平、脂滴形成和转录活性。
结果
GGOH增加了脂肪细胞系细胞中PPARγ的表达。GGOH还增强了罗格列酮（一种噻唑烷二酮类PPARγ激动剂）诱导的脂肪生成。
结论
GGOH诱导PPARγ表达并增强PPARγ激动剂在脂肪细胞系细胞中的生物学效应。
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