Liu Liang-Chih, Lin Yung-Hsiang, Lin Ying-Chao, Ho Chi-Tang, Hung Chao-Ming, Way Tzong-DER, Bau DA-Tian
Department of Surgery, Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan, R.O.C.
Department of Medicine, College of Medicine, China Medical University, Taichung, Taiwan, R.O.C.
In Vivo. 2018 Nov-Dec;32(6):1373-1379. doi: 10.21873/invivo.11389.
BACKGROUND/AIM: The banana flower is used for ameliorating urinary disturbance. However, there is limited evidence to support the efficacy or mechanism of action of banana flower against benign prostatic hyperplasia (BPH). In the present study, the anti-BPH activity and mechanisms of banana flower extracts were investigated in vitro and in vivo.
The banana flower extract is a water-soluble extract obtained by sonication. MTT assay was used to examine whether banana flower extract exhibited cytotoxic effects on BPH-1 cells. The effect of banana flower extract on cell-cycle distribution was examined by flow cytometry. The expression of cell-cycle-regulatory molecules was determined by western blot analysis. Testosterone propionate (TP)-induced rat model of BPH was used to evaluate the anti-BPH activity of banana flower extract in vivo.
Banana flower extract reduced epithelial cell line BPH-1 cell viability through cell-cycle arrest at G phase. Moreover, banana flower extract reduced the expression of cyclin D1 and cyclin-dependent kinase 6, while it increased the expression of p53 and p27. Interestingly, banana flower extract suppressed BPH-related inflammatory responses through suppressing cyclo-oxygenase-2 expression and prostaglandin E2 production. Finally, banana flower extract administered orally to male rats reduced prostatic weight and serum dihydrotestosterone level, and improved prostate gland morphology. High-performance liquid chromatography revealed that banana flower extract contains citric acid, taurine, pantothenic acid and nicotinic acid components. In summary, banana flower extract may be used as a therapeutic agent for BPH via anti-proliferative and anti-inflammatory activities.
背景/目的：香蕉花被用于改善排尿障碍。然而，关于香蕉花治疗良性前列腺增生（BPH）的疗效或作用机制，支持证据有限。在本研究中，对香蕉花提取物的抗BPH活性及其机制进行了体内和体外研究。
香蕉花提取物是通过超声处理获得的水溶性提取物。采用MTT法检测香蕉花提取物对BPH-1细胞是否具有细胞毒性作用。通过流式细胞术检测香蕉花提取物对细胞周期分布的影响。采用蛋白质免疫印迹分析测定细胞周期调节分子的表达。利用丙酸睾酮（TP）诱导的大鼠BPH模型评估香蕉花提取物在体内的抗BPH活性。
香蕉花提取物通过使细胞周期停滞在G期降低上皮细胞系BPH-1细胞的活力。此外，香蕉花提取物降低细胞周期蛋白D1和细胞周期蛋白依赖性激酶6的表达，同时增加p53和p27的表达。有趣的是，香蕉花提取物通过抑制环氧化酶-2的表达和前列腺素E2的产生来抑制与BPH相关的炎症反应。最后，对雄性大鼠口服香蕉花提取物可减轻前列腺重量、降低血清双氢睾酮水平，并改善前列腺形态。高效液相色谱分析显示，香蕉花提取物含有柠檬酸、牛磺酸、泛酸和烟酸成分。总之，香蕉花提取物可能通过抗增殖和抗炎活性用作BPH的治疗药物。
